Distribution of Endogenous N-fixing Microbes in Agriophyllum squarrosum Plants

doi:10.7522/j.issn.1000-694X.2015.00169

JOURNAL OF DESERT RESEARCH ›› 2016, Vol. 36 ›› Issue (4): 1056-1062.DOI: 10.7522/j.issn.1000-694X.2015.00169

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Distribution of Endogenous N-fixing Microbes in Agriophyllum squarrosum Plants

Li Jianfeng^1,2, Zhang Shuqin^1,2, Miao Yangyang², Huang Tingzhou¹, Zhang Xihui¹

1. Institute of Soil and Environment Bioremediation in Karst habitats, Guizhou Normal College, Guiyang 550018, China;
2. Key Laboratory for Grassland Ecosystem of Ministry of Education, Gansu Agricultural University, Lanzhou 730070, China

Received:2015-09-09 Revised:2015-11-09 Online:2016-07-20 Published:2016-07-20

Abstract

Abstract: Agriophyllum squarrosum is a moving sand-fixing plant which is adapted to growing in mobile dunes, and it has a high plant nitrogen accumulation. To investigate the distribution of nitrogen-fixing bacteria in A. squarrosum whose different parts of different growth stages N-fixing microbes number of distribution. The results indicated that: A. squarrosum endogenous plant nitrogen-fixing bacteria have a great number of distribution heterogeneity in time and space. In time distribution, the number of nitrogen-fixing bacteria in the roots of reproductive growth was significantly higher than others; and the number of nitrogen-fixing bacteria in the vegetative growth stage leaves was significantly higher than other periods. The spatial distribution of the number of different parts of the organization within N-fixing microbes main source in the plant photosynthetic products-both ends of the distribution of the library. In addition to the vegetative growth stage, nitrogen-fixing bacteria mainly distributed in leaves 3.8×10⁵ cfu·5g^-1 (FW) all showed significantly higher than other parts of the root, and mainly existed in the fibrous; In the part of upper ground, making vegetative growth period as a typical representative, N-fixing microbes more concentrated on the upper end of its morphology, such as the top of the blade and stems.

Key words: Agriophyllum squarrosum, endophytic N-fixing microbes, temporal and spatial distribution, standing dead litter

CLC Number:

Li Jianfeng, Zhang Shuqin, Miao Yangyang, Huang Tingzhou, Zhang Xihui. Distribution of Endogenous N-fixing Microbes in Agriophyllum squarrosum Plants[J]. JOURNAL OF DESERT RESEARCH, 2016, 36(4): 1056-1062.

References

[1] Baldani J I,Olivares F L,Hemerly,et al.Nitrogen-fixing endophytes: recent advances in the association with graminaceous plants grown in the tropics[M]//Elemerich C,KordorosiA,Newton W E.Biological Nitrogen Fixation for the 21st Century.Netherlands:Kluwer Academic publishers,1997,203-206.
[2] Dobereiner J.Nitrogen fixing bacteria of the genus Beijerinck Derx in the rhizosphere of sugar cane[J].Plant and Soil,1961,15:211-216.
[3] Csvalcante V A,Dobereiner J.A new acid-tolerant nitrogen-fixing bacteria associated with sugar Cane[J].Plant and Soil,1988,108:23-31.
[4] 王秀呈,曹艳花,唐雪,等.水稻内生固氮菌Herbaspirillum seropedicae DX35的筛选及其促生特性[J].微生物学报,2014,54(3):292-298.
[5] 季华,潘存德,周俊,等.荒漠灌木内生固氮菌对环境因子的适应性研究[J].中国沙漠,2011,31(4):942-947.
[6] 谭志远,彭桂香,徐培智,等.普通野生稻(Oryza rufipogon)内生固氮菌多样性及高固氮酶活性[J].科学通报,2009,54(13):1885-1893.
[7] 赵现伟,Javed C H,何玉梅,等.先锋牧草-香根草联合固氮菌多样性[J].微生物学报,2009,49(11):1430-1437.
[8] 韩梅,罗培宇,肖亦农,等.玉米内生固氮菌的分离鉴定及其促生长作用研究[J].沈阳农业大学学报,2010,41(1):94-97.
[9] 施振云,倪礼斌.联合固氮菌剂拌种对水稻秧苗性状和产量的影响[J].中国土壤与肥料,1997(6):43-44.
[10] 吴文钧.优良复合生物菌肥-固氮菌剂[J].新农业,2005,(9):30-31.
[11] 方华舟,左雪枝.稻田固氮解磷解钾菌筛选及其复合菌剂对土壤培肥作用[J].中国土壤与肥料,2014(2):82-87.
[12] 陈文新,陈文峰.发挥生物固氮作用减少化学氮肥用量[J].中国农业科技导报,2004,5(6):3-6.
[13] 王荣丽,廖宝文,何雪香,等.PGPB菌剂对5种红树小苗的野外接菌效应[J].东北林业大学学报,2015,43(1):103-106.
[14] Hashidoko Y.Autoxidation study of carotane sesquiterpenes possessing a non-conjugated 1,4-diene system[J].Journal of the Chemical Society,Perkin Transactions 1,1993,1(4):2351-2356.
[15] Tan Z,Peng G,Xu P,et al.Diversity and high nitrogenase activity of endophytic diazotrophs isolated from Oryzarufipogon Griff.[J].Chinese Science Bulletin,2009,54(16):2839-2848.
[16] 李华.平板菌落计数的改进方法[J].生物学通报,2006,41(1):51-51.
[17] 赵艳,张晓波.影响植物根际微生物区系之因素研究进展[J].中国农学通报,2007,23(8):425-430.
[18] 李剑峰,张淑卿,师尚礼,等.苜蓿内生根瘤菌分布部位与数量变化动态[J].中国生态农业学报,2009,17(6):1200-1205.
[19] 徐正金,罗明,王卫霞,等.3种典型荒漠灌木内生固氮菌及固氮酶基因nifH多样性分析[J].中国沙漠,2014,34(2):472-480.
[20] 李海军,颜震,朱希强,等.结冷胶发酵生产工艺的优化[J].食品与药品,2007,9:7-12.
[21] 胡桂萍,刘波,朱育菁,等.微生物食用胶-结冷胶的研究新进展[J].福建农业学报,2011,26(6):1123-1128.

Distribution of Endogenous N-fixing Microbes in Agriophyllum squarrosum Plants

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