Effects of short-term warming and precipitation reduction on soil microbial biomass carbon， nitrogen and enzyme activity in sandy grassland

doi:10.7522/j.issn.1000-694X.2021.00135

Journal of Desert Research ›› 2022, Vol. 42 ›› Issue (3): 274-281.DOI: 10.7522/j.issn.1000-694X.2021.00135

Effects of short-term warming and precipitation reduction on soil microbial biomass carbon， nitrogen and enzyme activity in sandy grassland

Huaihai Wang¹^,²(), Wenda Huang¹(), Yuanzheng He¹^,², Yayi Niu¹^,², Yuanzhong Zhu¹^,²

^1.Naiman Desertification Research Station，Northwest Institute of Eco-Environment and Resources，Chinese Academy of Sciences，Lanzhou 730000，China
^2.University of Chinese Academy of Sciences，Beijing 100049，China

Received:2021-07-02 Revised:2021-10-29 Online:2022-05-20 Published:2022-06-01
Contact: Wenda Huang

Abstract

Abstract:

In this study， we simulated climate change to explore the effects of short-term warming and precipitation reduction on soil microbial biomass carbon， nitrogen and enzyme activities in sandy grassland plant communities， and further revealed the response rules of soil microbial biomass carbon， nitrogen and enzyme activities in sandy grassland to short-term climate change. The results showed that：（1） Short-term warming and precipitation reduction had significant effects on soil microbial biomass carbon， nitrogen and enzyme activities. （2） Under natural temperature， soil MBC and MBN contents in precipitation decreased by 40% increased by 87.9% and 98.8%， respectively. S-ALPT activity was the lowest in precipitation decreased by 60%， which decreased by 32.8%. （3） Under the warming condition， soil MBC and MBN contents were the lowest when precipitation decreased by 40%， with a decrease of 25.67% and 48.16%， respectively. S-UE content was the highest， and the increase range was 20.42%. （4） Soil pH was positively correlated with the activities of three soil enzymes， negatively correlated with soil microbial biomass carbon and nitrogen. Soil microbial biomass carbon and nitrogen were negatively correlated with S-CL， and positively correlated with S-UE and S-ALPT.

Key words: warming, precipitation reduction, sandy grassland, microbial biomass carbon and nitrogen, soil enzyme activity

CLC Number:

Q938.1

Huaihai Wang, Wenda Huang, Yuanzheng He, Yayi Niu, Yuanzhong Zhu. Effects of short-term warming and precipitation reduction on soil microbial biomass carbon， nitrogen and enzyme activity in sandy grassland[J]. Journal of Desert Research, 2022, 42(3): 274-281.

Figures/Tables 7

References 44

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处理	土层 /cm	土壤机械组成/%						pH
处理	土层 /cm	2—1 mm	1—0.5 mm	0.5—0.25 mm	0.25—0.1 mm	0.1—0.05 mm	<0.05 mm	pH
T₀×W₀	0—10	0.21 ±0.14	0.34±0.11	28.47±1.22	55.68±2.85	10.14±1.69^a	4.78±1.38^a	8.04±0.25^a
T₀×W₀	10—20	0.19±0.04^a	0.36±0.07^a	28.44±1.16^a	55.92±3.58	10.32±2.90	4.50±1.06^a	7.78±0.22
T₀×W₂₀	0—10	0.21 ±0.06	0.38±0.92	27.30±0.91	56.58±0.93	12.31±1.39^b	3.26±0.87^b	7.74±0.27^b
T₀×W₂₀	10—20	0.06±0.01^b	0.25±0.05^bc	26.86±0.63^b	57.59±2.33	12.09±2.19	3.15±0.61^b	8.06±0.44
T₀×W₄₀	0—10	0.19±0.03	0.37±0.11	27.29±1.49	57.28±1.68	10.51±1.00^ac	4.25±0.65^abc	7.84±0.15^ab
T₀×W₄₀	10—20	0.07±0.03^b	0.31±0.07^ac	27.68±0.75^abc	57.53±1.67	11.21±0.73	3.33±0.39^b	8.13±0.34
T₀×W₆₀	0—10	0.20±0.05	0.42±0.10	27.83±1.63	56.21±1.25	10.58±1.11^abc	4.85±1.02^ac	7.69±0.11^bc
T₀×W₆₀	10—20	0.05±0.02^b	0.31±0.09^ac	28.61±1.47^ac	56.29±1.82	10.07±1.45	4.95±0.76^a	8.08±0.25
T×W₀	0—10	0.23±0.04^a	0.40±0.08^a	28.77±2.14^a	54.60±2.25	11.26±0.30^a	4.38±0.29^a	7.90±0.19
T×W₀	10—20	0.66±0.03	0.29±0.07	29.06±1.45^a	55.77±1.59	10.77±0.90	3.92±0.14^a	8.21±0.29
T×W₂₀	0—10	0.39±0.08^ac	0.33±0.09^ac	26.83±3.21^ac	54.69±2.43	13.20±1.07^ac	4.21±1.13^a	7.95±0.26
T×W₂₀	10—20	0.18±0.05	0.32±0.08	26.61±2.44^ac	56.15±1.48	12.78±1.99	3.78±0.71^a	8.19±0.33
T×W₄₀	0—10	0.47±0.24^bc	0.25±0.51^bc	24.50±0.38^bc	54.55±4.09	11.80±2.27^ac	8.28±2.06^b	7.98±0.36
T×W₄₀	10—20	0.12±0.07	0.30±0.06	26.63±1.55^ac	54.78±2.86	12.65±2.54	5.12±0.64^b	8.20±0.32
T×W₆₀	0—10	0.34±0.06^abc	0.31±0.04^abc	26.59±1.77^abc	54.52±2.06	14.11±3.08^bc	3.94±0.51^a	8.01±0.30
T×W₆₀	10—20	0.17±0.04	0.25±0.03	26.46±1.91^bc	56.38±1.16	13.12±2.09	3.28±0.31^a	8.16±0.40

处理	土层 /cm	土壤机械组成/%						pH
处理	土层 /cm	2—1 mm	1—0.5 mm	0.5—0.25 mm	0.25—0.1 mm	0.1—0.05 mm	<0.05 mm	pH
T₀×W₀	0—10	0.21 ±0.14	0.34±0.11	28.47±1.22	55.68±2.85	10.14±1.69^a	4.78±1.38^a	8.04±0.25^a
T₀×W₀	10—20	0.19±0.04^a	0.36±0.07^a	28.44±1.16^a	55.92±3.58	10.32±2.90	4.50±1.06^a	7.78±0.22
T₀×W₂₀	0—10	0.21 ±0.06	0.38±0.92	27.30±0.91	56.58±0.93	12.31±1.39^b	3.26±0.87^b	7.74±0.27^b
T₀×W₂₀	10—20	0.06±0.01^b	0.25±0.05^bc	26.86±0.63^b	57.59±2.33	12.09±2.19	3.15±0.61^b	8.06±0.44
T₀×W₄₀	0—10	0.19±0.03	0.37±0.11	27.29±1.49	57.28±1.68	10.51±1.00^ac	4.25±0.65^abc	7.84±0.15^ab
T₀×W₄₀	10—20	0.07±0.03^b	0.31±0.07^ac	27.68±0.75^abc	57.53±1.67	11.21±0.73	3.33±0.39^b	8.13±0.34
T₀×W₆₀	0—10	0.20±0.05	0.42±0.10	27.83±1.63	56.21±1.25	10.58±1.11^abc	4.85±1.02^ac	7.69±0.11^bc
T₀×W₆₀	10—20	0.05±0.02^b	0.31±0.09^ac	28.61±1.47^ac	56.29±1.82	10.07±1.45	4.95±0.76^a	8.08±0.25
T×W₀	0—10	0.23±0.04^a	0.40±0.08^a	28.77±2.14^a	54.60±2.25	11.26±0.30^a	4.38±0.29^a	7.90±0.19
T×W₀	10—20	0.66±0.03	0.29±0.07	29.06±1.45^a	55.77±1.59	10.77±0.90	3.92±0.14^a	8.21±0.29
T×W₂₀	0—10	0.39±0.08^ac	0.33±0.09^ac	26.83±3.21^ac	54.69±2.43	13.20±1.07^ac	4.21±1.13^a	7.95±0.26
T×W₂₀	10—20	0.18±0.05	0.32±0.08	26.61±2.44^ac	56.15±1.48	12.78±1.99	3.78±0.71^a	8.19±0.33
T×W₄₀	0—10	0.47±0.24^bc	0.25±0.51^bc	24.50±0.38^bc	54.55±4.09	11.80±2.27^ac	8.28±2.06^b	7.98±0.36
T×W₄₀	10—20	0.12±0.07	0.30±0.06	26.63±1.55^ac	54.78±2.86	12.65±2.54	5.12±0.64^b	8.20±0.32
T×W₆₀	0—10	0.34±0.06^abc	0.31±0.04^abc	26.59±1.77^abc	54.52±2.06	14.11±3.08^bc	3.94±0.51^a	8.01±0.30
T×W₆₀	10—20	0.17±0.04	0.25±0.03	26.46±1.91^bc	56.38±1.16	13.12±2.09	3.28±0.31^a	8.16±0.40

处理	df	MBC/(mg·kg^-1)		MBN/(mg·kg^-1)		MBC/MBN
处理	df	F	P	F	P	F	P
T	1	9.040	0.008	5.033	0.039	16.713	0.001
W	3	1.597	0.229	6.398	0.005	4.576	0.017
T×W	3	13.521	<0.001	14.336	<0.001	0.627	0.068

处理	df	MBC/(mg·kg^-1)		MBN/(mg·kg^-1)		MBC/MBN
处理	df	F	P	F	P	F	P
T	1	9.040	0.008	5.033	0.039	16.713	0.001
W	3	1.597	0.229	6.398	0.005	4.576	0.017
T×W	3	13.521	<0.001	14.336	<0.001	0.627	0.068

处理	df	S-UE/(U·g^-1)		S-CL/(U·g^-1)		S-ALPT/(U·g^-1)
处理	df	F	P	F	P	F	P
T	1	13.981	0.001	7.539	0.001	8.205	0.007
W	3	9.972	<0.001	0.701	0.558	13.309	<0.001
T×W	3	1.962	0.139	1.231	0.315	16.432	<0.001

Effects of short-term warming and precipitation reduction on soil microbial biomass carbon， nitrogen and enzyme activity in sandy grassland

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项目	pH	MBC	MBN	S-UE	S-CL	S-ALPT
MBC	-0.436^*
MBN	-0.194	0.155
S-UE	0.252	0.099	0.146
S-CL	0.226	-0.067	-0.066	-0.098
S-ALPT	0.143	0.065	0.383^*	0.182	-0.230
MBC/MBN	-0.174	0.414^*	-0.522^**	-0.344	-0.003	-0.194

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