Effects of seed soaking on the changes of soil Microorganism flora in the rhizosphere of Lanzhou lily

doi:10.7522/j.issn.1000-694X.2022.00059

Journal of Desert Research ›› 2022, Vol. 42 ›› Issue (3): 251-260.DOI: 10.7522/j.issn.1000-694X.2022.00059

Effects of seed soaking on the changes of soil Microorganism flora in the rhizosphere of Lanzhou lily

Yibin Li¹(), Guihong Shi²^,³, Guiying Shi²(), Hongyu Yang², Hui Li²

^1.College of Life Science，Sichuan Agricultural University，Ya'an 625000，Sichuan，China
^2.College of Horticulture，Gansu Agricultural University，Lanzhou 730070，China
^3.Danfeng County Agricultural and Rural Bureau，Danfeng 726200，Shaanxi，China

Received:2022-02-19 Revised:2022-04-06 Online:2022-05-20 Published:2022-06-01
Contact: Guiying Shi

Abstract

Abstract:

The purpose of this study was to evaluate the effects of seed surface disinfection on the rhizosphere soil microbial community structure of Lanzhou lily （Lilium davidii var. unicolor）， and to search for key bacteria and fungi that regulate plant growth. Next-generation sequencing technology （NGS） was used to analyse the changes of bacteria and fungi in plant rhizosphere soil after SD treatment （bulb seed soaking treatment）. The findings shows that： the SD treatment has increased soil bacterial abundance index （Chao1） significantly， and has changed the microbial community structure as well. It is significant that the relative abundances （RA） of the genus Penicillium have increased， while the genera Microascus and Chaetomium RA have decreased significantly. It is significant that the phylum Proteobacteria and its subset genus Devosia and Mesorhizobium and Pseudarthrobacter have decreased， while the genera Flavobacterium and Jatrophihabitans have increased. In conclusion， seed soaking induce changes in the community structure of fungi and bacteria in rhizosphere soil of lily seedlings. The enrichment of the beneficial bacteria Penicillium and its species PenicilliumAurantiogriseum，Flavobacterium and Jatrophihabitans， as well as the reduction of the harmful bacteria Devosia improve soil health and enhance lily seedlings growth.

Key words: Lanzhou lily, seed soaking, bacteria community structure, fungi community structure, growth and development

CLC Number:

Q938.1

Yibin Li, Guihong Shi, Guiying Shi, Hongyu Yang, Hui Li. Effects of seed soaking on the changes of soil Microorganism flora in the rhizosphere of Lanzhou lily[J]. Journal of Desert Research, 2022, 42(3): 251-260.

Figures/Tables 5

Table 1 Significance analysis of seed soaking treatment on the growth of Lanzhou lily plants and comprehensive growth and growth yields indicators

壮苗指数

（鲜）

壮苗指数（鲜）

根冠比（干）

壮苗指数

（干）

壮苗指数

（鲜）

根冠比（干）

鳞茎质量

经济产量

/(kg·hm^-2）

Fig.1 The distribution and diversity analysis of rhizosphere soil OTU after Lanzhou lily bulb seed soaking treatment： Fungus Venn diagram （A）， fungus Chaol， Shannon index diagram （B）， bacteria Venn diagram （C）， bacteria Chaol， Shannon index diagram （D）

Fig.2 The rhizosphere soil fungi PCA （A） and bacteria PCA （B） after Lanzhou lily bulb seed soaking treatment

Fig.3 The microorganism community structures of rhizosphere soil after soaking the seeds of Lanzhou lily bulb： fungal （A-E）， bacterial phyla （F-J）， and the genus Fusarium （K）

Fig.4 LEfSe analysis showing the relative abundance of fungal （A） and bacterial （B） communities in the rhizosphere soil after soaking of Lanzhou lily bulbs

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Effects of seed soaking on the changes of soil Microorganism flora in the rhizosphere of Lanzhou lily

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