| 生物土壤与生态 |
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| Preparation of Total DNA from Desert Plant Calligonum L. |
JIN Ling1,2, LI Ming1, ZHANG Yong3,4, BAI Lei3, AN Li-zhe1,3, CHEN Tuo1
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| 1.Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China; 2.Gansu Traditional Chinese Medicine College, Lanzhou 730000, China; 3.School of Life Science, Lanzhou University, Lanzhou 730000, China; 4.Department of Life Science of Hexi College, Zhangye 734000, Gansu, China |
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Abstract We introduced a better method which do best for the purity and concentration of total DNA of seven Calligonum species: Calligonum mongolicum Turcz. , C. junceum (Fisch. et Mey.) Endl., C. aphyllum (Pall.) G rke,C. rubicundum Bge., C. cordatum E. Kor. ex N. Pavl., C. densum Borszcz., C. arborescens Litv.. This method is an improved one upon material and method based on the traditional CTAB method. The result showed that it could effectively eliminate the affection of secondary materials to extracted total DNA from Calligonum, and is suitable for PCR of ISSR and other genetic research. Also, it provides a reliable experimental method for relevant researches on Calligonum.
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Received: 11 January 2006
Published: 20 May 2007
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[1]李安仁.中国植物志[M].北京:科学出版社,1998: 118-119.
[2]刘媖心. 沙漠植物志[M]. 北京:科学出版社, 1985: 331-439.
[3]陶玲,刘志学,何兴东.用于RAPD分析的沙拐枣DNA提取方法[J].中国沙漠,2001,21(3):300-302.
[4]高志海,崔建国,刘生龙,等.民勤沙区沙拐枣果实性状的变异性研究[J].中国沙漠, 1998,18(3): 244-248.
[5]彭鸿嘉,傅伯杰,陈利顶,等.甘肃民勤荒漠区植被演替特征及驱动力研究[J].中国沙漠, 2004,24(5): 628-633.
[6]杨自辉.民勤沙井子地区40a来荒漠植被变迁初探[J].中国沙漠,1999,19(4): 395-397.
[7]刘金荣,李艳,周静.沙拐枣果氨基酸分析[J].农垦医学,2001,23(5): 289-290.
[8]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DAN [J]. Nucleic Acids Research,1980, 8:4321-4325.
[9]曾杰,邹喻苹,白嘉雨,等.玩拗植物类群的总DNA制备[J].植物学报,2002,44(6):694-697.
[10]刘志学,陈妍珂. 沙漠植物DNA快速提取方法[J].中国沙漠,1997,17(3):274-279.
[11]张娟,张道远,尹林克.刚毛柽柳基因组DNA提取和RAPD反应条件探索[J].西北植物学报,2003,23（2）：253-256.
[12]丁晓东,吕柳新.从玩拗植物荔枝中提取基因组DNA技术的研究[J].应用与环境生物学报,2000,6(2):142-145.
[13]Wolfe A D, Xiang Q Y, Kephart S R. Assessing hybridization in natural population of Penstemon (Scrophulariaceae) using Hypervariable intersimple sequence repeat (ISSR) bands[J]. Mol Ecol, 1998, 7:1107-1125. |
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2007, Vol. 27 
