| 生物土壤与生态 |
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| Introduction of Soybean Gy3 Gene into Callus of Hedysarum scoparium by Corona Discharge and Plantlet Regeneration |
| GAO Jing1,2, Nari1,3, CHAO lu-meng1,3, XU Xiao1,3, Bagenna1,3 |
| 1.School of Physical Science and Technology, Inner Mongolia University, Hohhot 010021, China; 2.Inner Mongolia Meteorological Bureau, Hohhot 010051, China; 3.Key Laboratory of Ion Beam Bioengineering, Inner Mongolia University, Hohhot 010021, China |
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Abstract The pBI121 plasmid, which contains a copy of GUS gene and kanamycin resistance gene, was introduced into Hedysarum scoparium calli by corona discharge, and transient expression of GUS gene was detected and discovered in these receptor calli by histochemical localization reaction. The soybean Gy3 gene in Hedysarum scoparium transformation calli was screened by Kanamycin of 80 mg\5L-1, and 38 regenerated plants were obtained, and there were soybean Gy3 gene in 3 plants. PCR and RT-PCR detection showed that the Soybean Gy3 gene had been introduced into DNA of the regenerated plants of Hedysarum scoparium successfully.
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Received: 22 December 2010
Published: 20 September 2011
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2011, Vol. 31 
