| 生物土壤与生态 |
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| PCR-RFLP Analysis of Nodulation Gene nodA of Rhizobia from Ammopiptanthus mongolicus |
| BI Jiang-tao1,2,3, HE Da-han1, XIE Rui-mei2, WEI Ge-hong2 |
| 1.College of Agriculture, Ningxia University, Yinchuan 750012, China; 2.Microbial Research Center, Northwest Agriculture and Forestry University, Yanglin 712100, Shaanxi, China; 3.Ningxia Extension Service for Agricultural Techniques, Yinchuan 750001, China |
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Abstract Studies on relations between genotypes and phylogenic of 36 rhizobial strains isolated from Ammopiptanthus mongolicus were conducted by the methods of nodA PCR-RFLP and nodA sequencing. The results of PCR-RFLP clustering analysis showed that, at the similarity of 80%, the 36 strains are clustered with 2 groups within which there are 5 subgroups. The sequencing of the representative tested strains chosen from the identified different genotypes in the trials was conducted, and the analysis on phylogenic relations indicated that the tested strains of CCNWNX0117, CCNWNX0068, CCNWNX0083, CCNWNX0062, and CCNWNX0058 clustered with Mesorhizobium, and that the phylogenetic status is very close; meanwhile the tested strains of CCNWNX0089, CCNWNX0081, CCNWNX0064 clustered with Sinorizobium, the similarity of sequences is high. The studies indicated that the genetic diversity of nodA gene is relatively rich and has a broad adaptability to the potential hosts in nodulation.
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Received: 13 October 2008
Published: 20 July 2009
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2009, Vol. 29 
