乌拉特荒漠草原小针茅(Stipa klemenzii)群落土壤细菌多样性及垂直分布特征

doi:10.7522/j.issn.1000-694X.2016.00053

摘要/Abstract

摘要： 测定了乌拉特荒漠草原典型植被类型——小针茅（Stipa klemenzii）群落土壤细菌多样性及垂直分布特征。结果表明：乌拉特荒漠草原小针茅群落土壤细菌多样性的垂直分布表现出0~5 cm>10~20 cm>5~10 cm，其中表层（0~5 cm）土壤细菌多样性显著高于下层（5~10 cm和10~20 cm），5~10 cm和10~20 cm层之间差异不显著。土壤细菌在不同深度之间的群落相似度60%~70%。小针茅群落中土壤细菌分别归属于放线菌门（Actinobacteria）、芽单胞菌门（Gemmatimonadetes）、蓝细菌门（Cyanobacteria）、厚壁菌门（Firmicutes）、变形菌门（Proteobacteria）和酸杆菌门（Proteobacteria）。其中，Halospirulina sp.、Bacillus circulans和Lactobacillus pentosus是优势细菌（占总细菌的44.05%）。在荒漠草原小针茅群落中，土壤养分、机械组成和酸碱度共同影响着土壤细菌群落组成和分布格局，其中土壤pH值、全氮和黏粉粒含量（贡献率分别是24.28%、15.81%和10.60%）是影响细菌群落垂直分布的主要环境因子。

关键词: 荒漠草原, 小针茅(Stipa klemenzii), 土壤细菌多样性, 垂直分布, PCR-DGGE

Abstract: Stipa klemenzii community is the typical vegetation in Urad desert steppe. Our observation on soil bacterial diversity and vertical distribution in Stipa klemenzii community showed that:soil bacterial vertical distribution performed 0-5 cm > 10-20 cm > 5-10 cm, and the diversity in the upper layer (0-5 cm) was significantly higher than that in the lower layers (5-10 cm and 10-20 cm). The bacterial community similarities were within 60%-70% among the three layers. The sequences analysis indicated that the bacterial species belonged to different phylum, including Actinobacteria, Gemmatimonadetes, Cyanobacteria, Firmicutes, Proteobacteria and Proteobacteria. Halospirulina sp., Bacillus circulans and Lactobacillus pentosus were the three dominant bacterial species (their abundance occupied 44.05% of all the 16 species). Soil nutrient, texture and pH collaboratively influenced the bacterial community composition and distribution. Soil pH, total nitrogen content and silt and clay content are the key factors to determine soil bacterial community composition in the Stipa klemenzii community of desert steppe, contributing 24.28%, 15.18% and 10.60%, respectively.

Key words: desert steppe, Stipa klemenzii, soil bacterial diversity, vertical distribution, PCR-DGGE

中图分类号:

Q938.1

王少昆, 赵学勇, 贾昆峰, 高宝兰, 曲浩, 毛伟, 连杰, 陈敏, 朱阳春. 乌拉特荒漠草原小针茅(Stipa klemenzii)群落土壤细菌多样性及垂直分布特征[J]. 中国沙漠, 2016, 36(6): 1564-1570.

Wang Shaokun, Zhao Xueyong, Jia Kunfeng, Gao Baolan, Qu Hao, Mao Wei, Lian Jie, Chen Min, Zhu Yangchun. Soil Bacterial Diversity and Its Vertical Distribution in Stipa klemenzii Community of Urad Desert Steppe[J]. JOURNAL OF DESERT RESEARCH, 2016, 36(6): 1564-1570.

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