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中国沙漠  1995, Vol. 15 Issue (1): 96-99    
论文     
离子交换柱层析法分离鉴定同工酶的技术探讨
张利平1, 刘新民1, 陈靠山2
1. 中国科学院兰州沙漠研究所;
2. 南京农业大学农学系
TECHNICAL DISCUSSION ON ISOENZYME ISOLATION AND IDENTIFICATION WITH ION EXCHANGE CHROMATOGRAPHY METHOD
Zhang Liping1, Liu Xinmin1, Chen Kaoshan2
1. Institute of Desert Research, Chinese Academy of Sciences, Lanzhou, 730000;
2. Department of Agronomy, nan jing Agricultural University
 全文: PDF(251 KB)  
摘要: 本文介绍了离子交换柱层析法的基本原理、操作要点及其在分离鉴定没有特异染料显色的同工酶中的应用, 并时电泳法和离子交换柱层析法在分离同工酶中的应用范围和应用前景, 尤其对它们的优缺点进行了评价。
关键词: 离子交换层析同工酶    
Abstract: This paper described the basic principles and operating methods of ion-exchange chromatography and its application in the isoenzyme isolation and identification without special dye colouration,The advantages,disadvantages of th is technique and electrophoresis and their prospects and range of application to isoenzyme isolation and identification also were further discussed.
Key words: Ion-exchange chromatography    Isoenzyme
收稿日期: 1994-01-10
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引用本文:

张利平, 刘新民, 陈靠山. 离子交换柱层析法分离鉴定同工酶的技术探讨[J]. 中国沙漠, 1995, 15(1): 96-99.

Zhang Liping, Liu Xinmin, Chen Kaoshan. TECHNICAL DISCUSSION ON ISOENZYME ISOLATION AND IDENTIFICATION WITH ION EXCHANGE CHROMATOGRAPHY METHOD. JOURNAL OF DESERT RESEARCH, 1995, 15(1): 96-99.

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http://119.78.100.150:82/zgsm/CN/        http://119.78.100.150:82/zgsm/CN/Y1995/V15/I1/96

[1] 孙雪新, 康向阳. 胡杨天然群体过氧化物酶同工酶遗传变异初探[J]. 中国沙漠, 1991, 11(1): 30-35.