NaCl胁迫对骆驼蓬幼苗液泡膜H+-ATPase和H+-PPase活性的影响

摘要/Abstract

摘要： 研究了不同浓度NaCl胁迫对骆驼蓬幼苗Na⁺、K⁺含量及液胞膜H⁺-ATP酶(H⁺-ATPase)和H⁺-焦磷酸酶(H⁺-PPase)活性的影响。结果表明，NaCl胁迫浓度的增加使骆驼蓬幼苗根、叶中的K⁺含量下降，Na+含量和Na+/K⁺比及K⁺对Na⁺的吸收和运输选择性增高；根、叶液胞膜H⁺-ATPase和H⁺-PPase活性升高，H⁺-ATP酶耦联比率无显著变化；根液胞膜依赖ATP和PPi的质子泵活性及Na⁺/H⁺逆向转运活性先升后降，叶依赖ATP的质子泵活性及Na⁺/H⁺逆向转运活性升高，依赖PPi的质子泵活性先升后降。说明液泡膜H⁺-ATPase和H⁺-PPase驱动的质子泵和Na⁺/H⁺逆向转运活性对Na+在液泡内的积累及其骆驼蓬的耐盐性起重要作用。

关键词: 骆驼蓬, NaCl胁迫, 液泡膜H⁺-ATPase, 液泡膜H⁺-PPase, 耦联比率, 质子泵活性

Abstract: Effects of the different concentration of NaCl stress on Na⁺ and K⁺ contents, tonoplast H⁺-ATPase and H⁺-PPase activities in peganum harmala seedling leaves were studied. The results showed that with NaCl stress concentration increased, K⁺ content in roots and leaves was decreased, but Na⁺ content, Na⁺/K⁺ ratio, K⁺/Na⁺ selectivity, as well as tonoplast H⁺-ATPase and H⁺-PPase activities were all increased, the H⁺/substrate stoichiometry was significantly unchanged, the activities of the ATP-dependent proton pump and the Na⁺/H⁺ antiport in leaves were increased, the activities of PPi-dependent proton pump in leaves and ATP and PPi-dependent proton pump and Na⁺/H⁺ antiport of the tonolast vesicles in roots were all increased and then decreased with the increase of NaCl concentration. These results indicated that the activity of Na⁺/H⁺ antiport and proton pump driven by H⁺-ATPase and H⁺-PPase in tonoplast of P. harmala plays an important role in Na+ accumulation in vacuoles and salt tolerance.

Key words: Peganum harmala, NaCl stress, tonoplast H⁺-ATPase, tonoplast H⁺-PPase, stoichiometry, proton pump activity

中图分类号:

刘建新;胡浩斌;赵国林. NaCl胁迫对骆驼蓬幼苗液泡膜H⁺-ATPase和H⁺-PPase活性的影响[J]. 中国沙漠, 2008, 28(2): 274-279.

LIU Jian-xin;HU Hao-bin;ZHAO Guo-lin. Effects of NaCl Stress on Tonoplast H⁺-ATPase and H⁺-PPase Activities in Peganum harmala Seedlings[J]. JOURNAL OF DESERT RESEARCH, 2008, 28(2): 274-279.

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