离子交换柱层析法分离鉴定同工酶的技术探讨

摘要/Abstract

摘要： 本文介绍了离子交换柱层析法的基本原理、操作要点及其在分离鉴定没有特异染料显色的同工酶中的应用, 并时电泳法和离子交换柱层析法在分离同工酶中的应用范围和应用前景, 尤其对它们的优缺点进行了评价。

Abstract: This paper described the basic principles and operating methods of ion-exchange chromatography and its application in the isoenzyme isolation and identification without special dye colouration,The advantages,disadvantages of th is technique and electrophoresis and their prospects and range of application to isoenzyme isolation and identification also were further discussed.

Key words: Ion-exchange chromatography, Isoenzyme

张利平, 刘新民, 陈靠山. 离子交换柱层析法分离鉴定同工酶的技术探讨[J]. 中国沙漠, 1995, 15(1): 96-99.

Zhang Liping, Liu Xinmin, Chen Kaoshan. TECHNICAL DISCUSSION ON ISOENZYME ISOLATION AND IDENTIFICATION WITH ION EXCHANGE CHROMATOGRAPHY METHOD[J]. JOURNAL OF DESERT RESEARCH, 1995, 15(1): 96-99.

离子交换柱层析法分离鉴定同工酶的技术探讨

TECHNICAL DISCUSSION ON ISOENZYME ISOLATION AND IDENTIFICATION WITH ION EXCHANGE CHROMATOGRAPHY METHOD

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