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JOURNAL OF DESERT RESEARCH  2013, Vol. 33 Issue (2): 554-559    DOI: 10.7522/j.issn.1000-694X.2013.00076
    
ITS Sequence Analysis of Artemisia halodendron in Different Habitats Gradients
HUANG Wen-da, ZHAO Xue-yong, ZUO Xiao-an, LI Yu-qiang, LIAN Jie
Naiman Desertification Research Station, Cold and Arid Regions and Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China
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Abstract  

The aim of the present study is to investigate the difference between ITS sequences of Artemisia halodendron from different habitats gradients in Horqin Sandy Land, and the Artemisia depauperata is selected as outgroup. The results indicate that the total length of A. halodendron ITS is 696 bp, the lengths of the unaligned ITS-1 and ITS-2 sequences varied from 253 bp to 256 bp and 264 bp to 269 bp, respectively, the GC content of the ITS-1 and ITS-2 sequences ranged from 54.02% to 54.77% and 56.75% to 58.64%, respectively. It indicates that there is a high difference of the length and composition of sequence in ITS-1 than ITS-2. The genetic identities between ITS sequences of A. halodendron from 9 populations ranged from 85.7% to 99.7%. It indicates that there are some genetic differentiations between sequences. In the most parsimonious tree, most ITS sequences from A. halodendron showed two major clades: Clade Ⅰ and Clade Ⅱ, and Clade Ⅱ older than Clade Ⅰ, the order were subp4→subp1→subp2→subp3→subp8→subp6→subp7→subp9→subp5,and corresponding habitats orders were Inter-dune lowland→Semi-mobile dune→mobile dune→semi-fixed dune→fixed dune. It indicates that there is a closely related between the evolutionary processes of A. halodendron and desertification forming processes and ecological restoration processes of Horqin Sandy Land.

Key words:  Horqin Sandy Land      Artemisia halodendron      habitat gradients      ITS     
Received:  30 May 2012      Published:  23 July 2012
ZTFLH:  Q145  

Cite this article: 

HUANG Wen-da, ZHAO Xue-yong, ZUO Xiao-an, LI Yu-qiang, LIAN Jie. ITS Sequence Analysis of Artemisia halodendron in Different Habitats Gradients. JOURNAL OF DESERT RESEARCH, 2013, 33(2): 554-559.

URL: 

http://www.desert.ac.cn/EN/10.7522/j.issn.1000-694X.2013.00076     OR     http://www.desert.ac.cn/EN/Y2013/V33/I2/554

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