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| Cyanobacterial Diversity in Biological Soil Crusts of Sand-fixing Vegetation Regions |
| Bao Jingting1,2,3, Wang Jin1, Chen Cuiyun1 |
1. Laboratory of Plant Stress Ecophysiology and Biotechnology/Shapotou Desert Experiment and Research Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China;
2. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract The cyanobacterial diversity in Shapotou region of Tengger desert was investigated through a culture-independent approach by constructing cyanobacterial specific 16S rDNA clone libraries. Positive clones selected randomly were sequenced and analyzed for phylogenetic tree constructing. Several diversity index including Shannon-Weaver diversity index, Simpson index, Chao and ACE richness index were done to estimate differences of cyanobacterial community between two regions. The results showed that the cyanobacterial community of Shapotou region of Tengger desert consists of Oscillatoriales(64.55%), Chroococcales(1.82%), Nostocales(16.36%), Pleurocapsales(6.82%) and unclassified Cyanobacteria(10.45%). After 57 years of restoration, Chao and ACE richness index of cyanobacterial community in artificial sand-fixing vegetation region were distinctively smaller than those of the natural vegetation region(recovering for more than 100 years), and there are still statistically significant difference in cyanobacterial community structure between the artificial sand-fixing vegetation region and natural sand-fixing vegetation region, which indicates the restoration of microbial diversity of desert ecosystem is a very long process.
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Received: 07 December 2014
Published: 20 November 2015
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2015, Vol. 35 
