Diversity of bacterial communities in the rhizocompartments of Caragana in the Mu Us Desert

doi:10.7522/j.issn.1000-694X.2019.00121

Journal of Desert Research ›› 2020, Vol. 40 ›› Issue (4): 128-137.DOI: 10.7522/j.issn.1000-694X.2019.00121

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Diversity of bacterial communities in the rhizocompartments of Caragana in the Mu Us Desert

Ziyuan Zhou(), Minghan Yu(), Guodong Ding, Guanglei Gao, Yingying He

Key Laboratory of State Forestry Administration on Soil and Water Conservation / MOE Engineering Research Center of Forestry Ecological Engineering / Yanchi Ecology Research Station of the Mu Us Desert, School of Soil and Water Conservation, Beijing Forestry University, Beijing 100083, China

Received:2019-08-23 Revised:2019-12-24 Online:2020-08-20 Published:2020-09-01
Contact: Minghan Yu

Abstract

Abstract:

Based on 16S rRNA high-throughput genome sequencing, the bacterial diversity and structural variability in the rhizocompartments (i.e.root, rhizosphere soil, root zone soil and inter-shrub bulk soil) of two Caragana spp.(Caragana microphylla and Caragana korshinskii) in the Mu Us Desert are studied.In this study, one-way ANOVA and PCA analysis based on OTU level with Euclidean distance were performed on the Alpha diversity indeices and Beta diversity of bacterial communities in each rhizocompartments, respectively.Then we confirmed the reports on the niche differentiation of plant rhizocompartments, the diversity and structural composition of the bacterial community in the rhizocompartments of desert leguminous plants showed significant differences, with a hierarchical transition of the four rhizocompartment types from outside to inside.We found that desert leguminous plants showed significant hierarchical filtration and enrichment of soil microbial communities under shrubs (P<0.05).The stratified selection and enrichment of specific bacterial groups by the rhizocompartments of plant is one of the main reasons for the hierarchical variation of bacterial community structure and composition in different ecological niches under the shrub of Caragana.

Key words: desert, Caragana, soil bacteria, diversity, structural variability

CLC Number:

Q938.1

Ziyuan Zhou, Minghan Yu, Guodong Ding, Guanglei Gao, Yingying He. Diversity of bacterial communities in the rhizocompartments of Caragana in the Mu Us Desert[J]. Journal of Desert Research, 2020, 40(4): 128-137.

Figures/Tables 5

Table 1 General features of the high-throughput sequencing results(n = 5)

Alpha多样性指数	Chao1指数	Shannon指数	OTU数目	Good’s Coverage指数
CM-r	1 707.11±457.09^b	6.95 ± 0.64^b	1 024±276^b	96.98%±0.86%^a
CM-rs	2 790.39±319.51^a	9.44± 0.16^a	2 000±181^a	95.13%±0.74%^b
CM-rz	2 735.70±255.00^a	9.30± 0.28^a	1 915±186^a	95.24%±0.53%^b
Bulk	2 440.77±413.01^a	8.90± 0.41^a	1 728±278^a	95.76%±0.81%^b

Fig.1 Rhizocompartment drives the composition of the bacterial communities at the OTU level

Table 2 ANOSIM analysis of rhizocompartments

根系微域	R	P
CMr-CMrs	0.912	0.005
CMr-CMrz	0.974	0.010
CMrs-CMrz	0.100	0.200
Bulk-CMr	1.000	0.008
Bulk-CMrs	0.640	0.005
Bulk-CMrz	0.228	0.100
r-rs-rz-Bulk	0.480	0.001

Fig.2 Microbial community compositions in the four rhizocompartments of Caragana Fabr. at the phylum and order levels

Fig.3 Venn diagrams showing significant enrichment (A) and reduction (B) of the relative operational taxonomic unit (OTU) abundances in the three rhizocompartments compared to root zone soil. C, the bacteria genera corresponding to relatively enriched OTUs; D, the bacteria genera corresponding to relatively reduced OTUs

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Diversity of bacterial communities in the rhizocompartments of Caragana in the Mu Us Desert

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