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Journal of Desert Research ›› 2020, Vol. 40 ›› Issue (3): 193-200.DOI: 10.7522/j.issn.1000-694X.2020.00009

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Iron metabolism microbial composition and functional genes response to succession of biological soil crust

Wang Yansong1,3, Liu Yubing1,2, Wang Zengru1,2, Zhao Lina1,3, Qi Jinghua1,3, Zhang Wenli1,3   

  1. 1. Shapotou Desert Research and Experimental Station, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China;
    2. Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China;
    3. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2019-10-24 Revised:2020-03-09 Online:2020-05-20 Published:2020-06-09

Abstract: Based on the microbial genomics microarray GeoChip 5.0 technology, the BSC of artificial sand-fixation vegetation in southern margin of Tengger desert was selected as the research object. Fungi participate in the iron uptake and transport processes of BSC, archaea participate in the iron transport and storage processes, while bacteria play dominant roles in all three processes of iron metabolism. The microbial species composition of iron metabolism function at phylum level did not respond significantly to succession, and the iron metabolism processes are mainly regulated by Proteobacteria. The functional gene intensities of iron metabolism to increase significantly and the three types of iron metabolism processes to reach the highest functional level should take 61 years. The main functional gene regulating BSC iron absorption process is ferrous oxidase protein encoding gene iro; the main functional gene regulating the iron transport process of prokaryote is ferric dihyroxybenzoylserine outer membrane transporter coding gene cirA and Fe transporter permease subunit coding gene feoB, the fungal iron transport process mainly relies on the high affinity of iron-containing cell transporters and ferric oxidase; the main functional gene regulating iron storage process is stationary phase nucleoid protein encoding gene dps. At the end of the BSC succession phase, the significant increase in the intensity of the aforementioned iron metabolism functional genes promoted the iron metabolism potential of the microorganisms. It was proved that the restoration of microbial iron metabolism potential in the process of vegetation restoration in arid and semi-arid desert ecosystems takes a long time.

Key words: biological soil crust(BSC), Tengger Desert, iron uptake, iron transport, iron storage, GeoChip 5.0

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