生物土壤与生态 |
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Characters of Ions Accumulation in Reaumuria soongorica Callus under Salt Stress |
TAN Hui-juan, JIA Rong-liang, LIU Yu-bing, ZHAO Xin, LI Xin-rong |
Shapotou Desert Experimental Research Station/Extreme Stress Resitance and Biotechnology Laboratory, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China |
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Abstract The ability to selectively absorb Na+, K+, Ca2+, and Cl- represents the salt tolerance of plants. In this study we stressed desert plant Reaumuria soongorica callus on Murashige and Skoog medium with 0~250 mM NaCl solution for 14 days, and then Na+, K+, Ca2+, Cl- contents in tissue of R. soongorica were measured. The result showed that low concentration of NaCl was required to promote growth of R. soongorica, and the maximum relative growth rate of R. soongorica was observed at 100 mM NaCl, whereas higher concentrations of NaCl (200 and 250 mM) inhibited growth. Under NaCl stress the K+, Ca2+content in R. soongorica callus decreased and the Na+, Cl- content increased and the decrement and increment increased as the NaCl concentration increased. When NaCl concentration was low the selectivity of R. soongorica for Na+, K+, Ca2+, Cl- absorption and transport and the capacity of their ion accumulation strengthened, and this was conducive to avoiding the disorder of ion metabolism and alleviating the damage of NaCl stress.
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Received: 20 February 2010
Published: 20 November 2010
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