荒漠植物红砂在持续干旱胁迫下的光保护机制研究

摘要/Abstract

摘要： 红砂（Reaumuria soogorica）是一种广泛分布于中国半荒漠地区的多年生半灌木,自然生境下具有很强的耐旱、耐高温和高辐射的能力。本实验在人为控制水分进行持续干旱胁迫的过程中,通过测定红砂的气体交换和叶绿素荧光参数的日变化来研究红砂干旱胁迫下的光保护机制。结果表明,土壤相对含水量下降的过程中,红砂叶片水势也相应地下降;净光合速率（Pn）的日变化由‘双峰型’逐渐趋向‘单峰型’;水分利用效率（WUE）随着干旱程度的增加而升高,植物接近休眠时下降;净光合速率 (Pn)、PSⅡ最大光化学效率 (Fv/Fm)和PSⅡ非循环式电子传递效率(ΦPSⅡ)在中午都明显降低;干旱胁迫下初始荧光(F0)出现明显的升高,日变化趋势为先上升后下降。说明红砂在干旱期间,采取了依赖于叶黄素循环的热能耗散和PSⅡ反应中心可逆失活两种光抑制的保护机制来度过干旱维持生存。

关键词: 红砂, 干旱胁迫, 净光合速率, 叶绿素荧光, 光抑制

Abstract: Reaumuria soogorica (pall.) Maxim, a perennial desert semi-shrub widely found in semi-arid environments of China, shows strong tolerance in drought, high temperature and intense radiation in its natural habitat. In present study, the photoprotective mechanism of Reaumuria Soogorica was investigated by analyzing diurnal variation of gas exchange and chlorophyll fluorescence parameters during progressive soil drying. The results showed that: with the soil water content decreased, the leaf water potential of Reaumuria Soogorica declined; the diurnal patterns of net photosynthesis rate (Pn) changed from two-peaks' to one-peak'; the leaf water use efficiency (WUE) increased gradually under drought stress and declined when the plant approaching dormancy; the net photosynthesis rate (Pn), the primary maximum photochemical efficiency of PSⅡ(Fv/Fm) and the quantum efficiency of non-cyclic electron transport of PSⅡ(ΦPSⅡ) decreased obviously at noon; the diurnal variations of minimal chlorophyll fluorescence (F0) significantly increased at first and then decreased. These indicated that the two photo inhibition mechanisms, the xanthophylls cycle-dependent thermal energy dissipation and the reversible inactivation of PSⅡ reaction center under drought stress, are used by Reaumuria Soogorica to survive the high drought.

Key words: Reaumuria Soogorica, drought stress, net photosynthesis rate, chlorophyll fluorescence, photo inhibition

中图分类号:

Q945.78

周生荟;刘玉冰*;谭会娟;王进. 荒漠植物红砂在持续干旱胁迫下的光保护机制研究[J]. 中国沙漠, 2010, 30(1): 69-73.

ZHOU Sheng-hui;LIU Yu-bing;TAN Hui-juan;WANG Jin. The Photoprotective Mechanism of Desert Plant Reaumuria soogorica under Progressive Soil Drying[J]. JOURNAL OF DESERT RESEARCH, 2010, 30(1): 69-73.

参考文献

[1]Mittler R,Merquiol E,Hallak-Herr E,et al.Living under a 'dormant' canopy:a molecular acclimation mechanism of the desert plant Retama raetam[J].The Plant Journal,2001,25 (4):407-415.
[2]Guo W,Li B,Huang Y,et al.Effects of different water stresses on eco-physiological characteristics of Hippophae rhamnoides seedlings[J].Acta Botanica Sinica,2003,45(10):1238-1244.
[3]Griffiths H,Parry M.Plant responses to water stress[J].Annals of Botony,2002,89:801-802.
[4]Chaitanya K,Jutur P,Sundar D,et al.Water stress effects on photosynthesis in different mulberry cultivars[J].Plant Growth Regulation,2003,40:75-80.
[5]Boyer T,Wong S,Farquhar G.CO2 and water vapour exchange across the leaf cuticle (epidermis) at various water potentials[J].Plant Physiology,1997,114:185-189.
[6]Lawlor D.Limitation to photosynthesis in water-stressed leaves:stomata vs metabolism and the role of ATP[J].Annals of Botoby,2002,89:871-885.
[7]Cooper K,Farrant J.Recovery of the resurrection plant Craterostigma wilmsii from desiccation:protection versus repair[J].Journal of Experimental Botany,2002,53:1805-1813.
[8]Oliver M.Desiccation tolerance in vegetative plant cells[J].Physiologia Plantarum,1996,97:779-787.
[9]Maroco J,Pereira J,Chaves M.Stomatal responses to leaf-to-air vapour pressure deficit in sahelian species[J].Australian Journal of Plant Physiology,1997,24:381-387.
[10]Balaguer L,Pugnaire F,Mart nez-Ferri E,et al.Ecophysiological significance of chlorophyll loss and reduced photochemical efficiency under extreme aridity in Stipa tenacissima L[J].Plant and Soil,2002,240:343-352.
[11]Zhao C,Wang G.Effects of Drought stress on the photoprotection in Ammopiptanthus mongolicus leaves[J].Acta Botanica Sinica,2002,44:1309-1313.
[12]Demming-Adams B,Adams WW Ⅲ.Photoprotection and other responses of plants to high light stress[J].Annual Review of Plant Physiology and Plant Molecular Biology,1992,43:599-626.
[13]Donald R.When there is too much light[J].Plant Physiology,2001,125:29-32.
[14]Niyogi K.Photoprotection revisited:Genetic and molecular approaches[J].Annual Review of Plant Physiology and Plant Molecular Biology,1999,50:333-359.
[15]刘玉冰,赵昕,谭会娟.荒漠植物红砂愈伤组织诱导与增殖研究[J].中国沙漠,2008,28(2):255-257.
[16]王万鹏,朱恭,李正平.红砂育苗的土壤水分管理初步研究[J].中国沙漠,2006,26(4):695-995.
[17]李秀玲,陈健,王刚.西北地区红砂种群ISSR遗传变异的空间自相关分析[J].中国沙漠,2008,28(3):964-969.
[18]肖生春,肖洪浪,宋耀选.荒漠植被红砂水热响应的年轮学研究[J].中国沙漠,2006,26(4):548-552.
[19]刘玉冰,张腾国,安黎哲,等.红砂正常和脱水组织中总RNA提取的改进CTAB法[J].中国沙漠,2006,26(4):600-603.
[20]Liu Y B,Zhang T G,Li X R,et al.Protective mechanism of desiccation tolerance in Reaumuria soongorica:leaf abscission and sucrose accumulation in the stem[J].Science in China(Series C),2007,50（1）:15-21.
[21]Liu Y,Wang G,Liu J,et al.Anatomical,morphological and metabolic acclimation in the resurrection plant Reaumuria soongorica during dehydration and rehydration[J].Journal of Arid Evironments,2007,70:183-194.
[22]Mao Z,Jiang H,Wang Y,et al.Water balance of birch and larch leaves and their resistance to short and progressive soil drought[J].Russ Journal of Plant Physiology,2004,51:697-701.
[23]许大全,张玉忠,张荣铣.植物光合作用的光抑制[J].植物生理学通讯,1992,28(4):237-243.
[24]Larcher W.Plant Physiological Ecology[M].New York:Springer-Verlag,1995.
[25]Lawlor D,Cornic G.Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants[J].Plant Cell and Environment,2002,25:275-294.
[26]Niyogi K.Photoprotection revisited:genetic and molecular approaches[J].Annual Review of Plant Physiology and Plant Molecular Biology,1999,50:333-359.
[27]Baker N.A possible role for photosystemⅡ in environmental perturbations of photosynthesis[J].Plant Physiology,1991,81:563-57.
[28]许大全,徐宝基,沈允钢.C3植物光合效率的日变化[J].植物生理学报,1990,16(1):1-5.
[29]武海,张树源,许大全,等.珊瑚树叶片叶绿素荧光非光化学猝灭的日变化和季节变化[J].植物生理学报,1997,23(2):145-150.
[30]Lambers H,Chapin Ⅲ F,Pons T.Plant Physiological Ecology[M].New York:Springer-Verlag,1998.