荒漠地区两种生态型芦苇叶片的抗氧化生理特性分析

摘要/Abstract

摘要： 以生长于腾格里沙漠南缘的沙丘芦苇（沙芦,DR）和戈壁盐湖芦苇（盐芦,GSR）为研究对象,以水生芦苇（SR）为对照材料,研究荒漠地区两种生态型芦苇在各自自然生境下改变体内还原状态的抗氧化机制。结果表明,沙芦和盐芦的脂质过氧化水平高于水芦,过氧化氢和一氧化氮含量也高,类胡萝卜素与叶绿素的比率增加,且盐芦高于沙芦。这表明沙芦和盐芦的体内氧化水平高于水芦,盐芦的氧化程度更高。抗氧化系统酶活性的响应分析表明,沙芦和盐芦叶片中总抗氧化能力大于水芦,超氧化物歧化酶、抗坏血酸过氧化物酶和一氧化氮合酶活性也高于水芦,但过氧化氢酶和谷胱甘肽还原酶活性没有明显的差异,而过氧化物酶活性却比水芦低。与质膜渗透调节能力相关的Na+/K+-ATP酶和Ca2+/Mg2+-ATP酶活性在盐芦中明显高于沙芦和水芦。这表明芦苇在干旱和盐渍条件下忍受诱导的次级氧化胁迫的能力提高。

关键词: 荒漠植物, 芦苇, 生态型, 抗氧化生理

Abstract: The aim of the present study is to investigate the anti-oxidative physiology in cellular redox environment of two ecotypes of reed (Phragmites communis Trin.) inhabiting in the desert region of China. Dune reed (DR) and Gobi salt reed (GSR) are selected from the Tengger Desert of northwest China, and the swamp reed (SR) is selected as control. The results indicate that the level of lipid peroxidation, the contents of hydrogen peroxide and nitric oxide and the ratio of carotenoids/chlorophyll are high in DR and GSR, and the levels in GSR are higher than in DR. It indicates that there is a high level of oxidation in GSR and DR, especially in GSR. The antioxidant defense system analysis show that DR and GSR possess higher activities of superoxide dismutase, ascorbate peroxidase and nitric oxide synthase than SR; Compared with SR, there is no significant differences in the activities of catalase and glutathione reductase for DR and GSR, and activity of peroxidase in DR and GSR is obviously lower than those in SR. Na+/K+-ATPase and Ca2+/Mg2+-ATPase, which relate to the ability of osmotic regulation of membrane, show a higher activities in GSR than those in DR and SR. These results suggest that the adaptation to saline or drought of common reed contributes to the high resistance to the oxidative stress related to apperceive environmental conditions.

Key words: desert plant, reed (Phragmites communis Trin.), ecotype, anti-oxidative physiology

刘玉冰;李新荣;谭会娟;李小军. 荒漠地区两种生态型芦苇叶片的抗氧化生理特性分析[J]. 中国沙漠, 2011, 31(2): 277-281.

LIU Yu-bing;LI Xin-rong;TAN Hui-juan;LI Xiao-jun. Leaf Anti-oxidative Physiology of Two Ecotypes of Reed in the Desert Regions, China[J]. JOURNAL OF DESERT RESEARCH, 2011, 31(2): 277-281.

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