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中国沙漠 ›› 2012, Vol. 32 ›› Issue (6): 1669-1673.

• 生物与土壤 • 上一篇    下一篇

盐胁迫对濒危植物长叶红砂抗氧化系统的影响

薛 焱1,2, 王迎春1*, 王同智1   

  1. 1.内蒙古大学 生命科学学院, 内蒙古 呼和浩特 010021;
    2.包头医学院, 内蒙古 包头 014060
  • 收稿日期:2012-05-26 修回日期:2012-06-08 出版日期:2012-12-20 发布日期:2012-06-08

Responses of Antioxidant System of an Endemic Halophyte Reaumuria trigyn to NaCl Stress

XUE Yan1,2, WANG Ying-chun1, WANG Tong-zhi1   

  1. 1.College of Life Science, Inner Mongolia University, Hohhot 010021, China; 2.Baotou Medical College, Baotou 014060, Inner Mongolia, China
  • Received:2012-05-26 Revised:2012-06-08 Online:2012-12-20 Published:2012-06-08

摘要:

以长叶红砂(Reaumuria trigyna)幼苗为研究对象,测定了不同浓度NaCl溶液胁迫30 d的长叶红砂叶片抗氧化特征参数和自由基及膜脂过氧化指标含量,以探讨长叶红砂抗氧化系统对盐胁迫的适应机制。结果显示,高盐胁迫下长叶红砂叶片O-2的产生速率显著上升,丙二醛(MDA)含量和相对电导率都在低盐浓度下降低,高盐浓度下随着盐浓度的增加而增加;长叶红砂叶片超氧化物歧化酶(SOD)和过氧化物酶(POD)活性随盐胁迫加剧而显著提高,过氧化氢酶(CAT)的活性变化较小;随盐浓度的提高谷胱甘肽的基本含量显著提高,抗坏血酸盐的含量在降低。研究表明,长叶红砂叶片中SOD、POD和谷胱甘肽在防御盐胁迫引起的氧化性损害中起到了显著保护作用。

关键词: 长叶红砂, 盐胁迫, 抗氧化系统

Abstract:

Reaumuria trigyna were cultivated in nutrient solutions at five NaCl levels(0, 100, 200,300, 400 mmol·L-1)for the investigation of plant antioxidant activities and active oxygen metabolism. Results show O-2 production rate significantly increases with NaCl concentration increase, and MDA and the relative electrical conductivity decrease first and then increase as NaCl concentration increases; the activities of SOD and POD significantly increase and CAT activity changes little as NaCl concentration increases; with increase of NaCl concentration, glutathione significantly increases, and ascorbic acid salt content decreases. The research revealed that SOD, CAT and glutathione are important components of antioxidant defense system for salt tolerance of R. trigyna.

Key words: Reaumuria trigyna, NaCl stress, antioxidant system

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