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PEG胁迫对胡枝子幼苗叶片生理特性的影响

马彦军1, 马 瑞1, 曹致中2, 李 毅1*   

  1. 1.甘肃农业大学 林学院, 甘肃 兰州 730070;
    2.甘肃农业大学 草业学院, 甘肃 兰州 730070
  • 收稿日期:2012-03-09 修回日期:2012-06-24 出版日期:2012-12-20 发布日期:2012-06-24

Effects of PEG Stress on Physiological Characteristics of the Lespedeza Seedlings Leaves

MA Yan-jun1, MA Rui1, CAO Zhi-zhong2, LI -Yi1   

  1. 1.College of Forestry, Gansu Agricultural University, Lanzhou 730070, China;
    2.Pratacultural College of Gansu Agricultural University, Lanzhou 730070, China
  • Received:2012-03-09 Revised:2012-06-24 Online:2012-12-20 Published:2012-06-24

摘要:

采用PEG处理人工模拟水分胁迫方法,研究了胡枝子属5种植物2 a生实生苗的抗旱性,并采用主成分分析法和隶属函数法对其抗旱性进行综合评价。结果表明,随着PEG处理浓度的增加,5种胡枝子叶片含水量、相对含水量、自由水和束缚水呈下降趋势;阴山胡枝子和截叶胡枝子的自然水分饱和亏随PEG浓度增加而增大,其余3种胡枝子自然水分饱和亏在PEG浓度为12%时达到最大;SOD、POD和CAT 3种酶活性随PEG浓度增大而逐渐升高,达到峰值后开始下降;MDA含量不断上升;可溶性蛋白质含量先增后降再增;叶片内游离脯氨酸含量持续增大;叶绿素a、b和总叶绿素含量先升后降;5种胡枝子相对电导率总体为持续增大。根据主成分分析对各胡枝子12个指标的隶属函数值进行计算,并求平均值,评价其抗旱顺序。抗旱性由强到弱的顺序为达乌里胡枝子、牛枝子、尖叶胡枝子、截叶胡枝子和阴山胡枝子。

关键词: 胡枝子, PEG, 隶属函数, 抗旱性

Abstract:

The drought resistance of 5 kinds of 2-year Lespedeza seedlings was studied in an artificial drought stress experiment with PEG(polyethyleneglycol) treatment, and was evaluated by the principal component analysis and the membership function. With the increase of PEG concentration, the leaf water content, relative water content, free water content and conventional irreducible water content decreased for all the 5 kinds of Lespedeza; the critical water saturation deficiency increased with the PEG concentration increase in L. inschanica (Maxim.) Schindl and L. cuneata G.don, the other three came to the maximum when the PEG concentration was 12%. The activity of SOD, POD and CAT gradually increased with increase of PEG concentration, and began to decline when the activity came to the peak; MDA content increased gradually; Soluble protein content increased firstly, then decreased, and increased after that; Free proline content in leaves continuously increased; Chlorophyll a, b and total chlorophyll contents increased firstly and then decreased; Relative electric conductivity of all five kinds of Lespedeza continuously increased. According to the principal component analysis results, the water content, relative water content, critical water saturation deficiency, free water content, conventional irreducible water content and MDA, Chlorophyll a, b and total chlorophyll contents, SOD, SP and Pro were chosen to assess the drought-resistance of the 5 kinds of Lespedeza by membership function. Results showed that the drought resistance of 5 kinds of Lespedeza obeyed the following order: L. daurica (Laxm.)Schindl>L. potaninii Vass>L. juncea(Linnf.) Pers>L. cuneata G.don>L. inschanica(Maxim.) Schindl.

Key words: Lespedeza, PEG treatment, membership function, drought resistance

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