3种荒漠植物幼苗生长和光合生理对氮增加的响应

摘要/Abstract

摘要： 选取荒漠地区广泛分布的3种多年生草本植物骆驼刺（Alhagi sparsifolia）、骆驼蹄瓣（Zygophyllum fabago）和盐生车前（Plantago maritima）,研究其生长和光合生理对氮增加的响应。结果表明: ①中等浓度和高浓度氮处理均能够促进3种植物生物量的增加。施氮浓度增加后,骆驼刺的根重和根长均增加,但是骆驼蹄瓣和盐生车前却降低。随着氮浓度增加,冠根比（S/R）亦增加,说明氮沉降增加能够促进生产力尤其是地上生产力增加。②随着氮浓度的增加, 植物的净光合速率（包括最大净光合速率）、叶绿素和可溶性蛋白增加。骆驼刺和盐生车前的瞬时水分利用效率表现为降低,而骆驼蹄瓣则表现为增加。3种植物可溶性糖含量随氮浓度的增加而减少。3种植物的光合生理特征响应能够很好地与生长相对应,在光合生理受到氮素营养限制时,其植物的生长较慢。当解除营养限制时,光合增加、可溶性糖减少,植物生物量增加。

关键词: 氮沉降, 生物量, 净光合速率, 叶绿素, 可溶性糖

Abstract:

An impact of nitrogen input on growth and photosynthetic physiology of plant species seedlings was analyzed with three desert species, Alhagi sparsifolia, Zygophyllum fabago and Plantago maritime, as examples. The median-and high-concentration input could promote the increase of biomass of these three desert species seedlings. With increase of nitrogen input, the root growth of A. sparsifolia increased, but that of Z. fabago and P. maritime did not. The shoot/root ratio of these three desert species all increased with the increase of nitrogen input, and it suggested that the nitrogen deposition could increase the productivity of plant, especially the aboveground part. With increase of nitrogen input, the net photosynthetic rate, contents of chlorophyll and soluble protein and soluble sugar of these three desert species also increased, but the water use efficiency of A. sparsifolia and P. maritima decreased and that of Z. fabago increased.

Key words: nitrogen deposition, biomass, net photosynthetic rate, chlorophyll, soluble sugar

中图分类号:

Q945

周晓兵;张元明*;王莎莎;张丙昌;张静;. 3种荒漠植物幼苗生长和光合生理对氮增加的响应[J]. 中国沙漠, 2011, 31(1): 82-89.

ZHOU Xiao-bing;ZHANG Yuan-ming;WANG Sha-sha;ZHANG Bing-chang;ZHANG Jing;. Effect of Nitrogen Input on Growth and Photosynthetic Physiology of Three Desert Species Seedlings[J]. JOURNAL OF DESERT RESEARCH, 2011, 31(1): 82-89.

参考文献

[1]Vourlitis G L,Zorba G.Nitrogen and carbon mineralization of semi-arid shrubland soil exposed to long-term atmospheric nitrogen deposition[J].Biology and Fertility of Soils,2007,43(5):611-615.
[2]Stursova M,Crenshaw C L,Sinsabaugh R L.Microbial responses to long-term N deposition in a semiarid grassland[J].Microbial Ecology,2006,51(1):90-98.
[3]Baez S,Fargione J,Moore D I,et al.Atmospheric nitrogen deposition in the northern Chihuahuan desert: Temporal trends and potential consequences[J].Journal of Arid Environments,2007,68(4):640-651.
[4]李德军,莫江明,方运霆,等.模拟氮沉降对南亚热带两种乔木幼苗生物量及其分配的影响[J].植物生态学报,2005,29(4):543-549.
[5]Schwinning S,Starr B I,Wojcik N J,et al.Effects of nitrogen deposition on an arid grassland in the Colorado plateau cold desert [J].Rangeland Ecology & Management,2005,58(6):565-574.
[6]Compton J E,Watrud L S,Porteous L A,et al.Response of soil microbial biomass and community composition to chronic nitrogen additions at Harvard forest [J].Forest Ecology and Management,2004,196(1):143-158.
[7]Zavaleta E S,Shaw M R,Chiariello N R,et al.Additive effects of simulated climate changes,elevated CO2,and nitrogen deposition on grassland diversity[J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(13):7650-7654.
[8]吕超群,田汉勤,黄耀.陆地生态系统氮沉降增加的生态效应[J].植物生态学报,2007,31(2):205-218.
[9]张燕,崔学民,樊明寿.大气氮沉降及其对草地生物多样性的影响[J].草业科学,2007,24(7):12-17.
[10]Brooks M L.Effects of increased soil nitrogen on the dominance of alien annual plants in the Mojave Desert[J].Journal of Applied Ecology,2003,40(2):344-353.
[11]Fenn M E,Baron J S,Allen E B,et al.Ecological effects of nitrogen deposition in the western United States[J].BioScience,2003,53(4):404-420.
[12]Hooper D U,Johnson L.Nitrogen limitation in dryland ecosystems:Responses to geographical and temporal variation in precipitation[J].Biogeochemistry,1999,46(1):247-293.
[13]胡明芳,田长彦,吕昭智,等.氮肥施用量对新疆棉花产量及植株和土壤中硝态氮含量的影响[J].西北农林科技大学学报,2006,34(4):63-68.
[14]李俊义,刘荣荣.新疆棉区棉花氮肥适宜用量和施用时期研究[J].中国棉花,1999,26(4):24-26.
[15]Aber J D,Nadelhoffer K J,Steudler P,et al.Nitrogen saturation in Northern Forest Ecosystems[J].BioScience,1989,39(6):378-386.
[16]马旭,田长彦,冯固,等.新疆农田投入化肥时空变化及趋势分析 [J].干旱区地理,2006,29(3):439-444.
[17]Xu M,Lu A H,Xu F,et al.Seasonal chemical composition variations of wet deposition in Urumchi,Northwestern China[J].Atmospheric Environment,2008,42(5):1042-1048.
[18]Wang X Q,Jiang J,Wang Y C,et al.Responses of ephemeral plant germination and growth to water and heat conditions in the southern part of Gurbantunggut Desert[J].Chinese Science Bulletin,2006,51:110-116.
[19]龚吉蕊,赵爱芬,苏培玺,等.黑河流域几个主要植物种光合特征的比较研究[J].中国沙漠,2005,25(4):587-592.
[20]贾荣亮,周海燕,谭会娟,等.超旱生植物红砂与珍珠光合生理生态日变化特征初探[J].中国沙漠,2006,26(4):631-636.
[21]张景光,周海燕.沙坡头地区一年生植物的生理生态特性研究[J].中国沙漠,2002,22(4):350-353.
[22]Prioul J F,Chartier P.Partitioning of transfer and carboxylation components of intracellular resistance to photosynthetic CO2 fixation:A critical analysis of the methods used[J].Annals of Botany,1977,41:789-800
[23]Lichtenthaler H K,Wellburn A R.Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents[J].Biochemical Society Transactions 1983,11:591-592.
[24]Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding[J].Analytical Biochemistry,1976,72:248-254.
[25]Dubois M,Gilles K A,Hamilton J K,et al.Colorimetric method for determination of sugars and related substances[J].Analytical Chemistry,1956,29:350-356.
[26]Patterson T B,Guy R D,Dang Q L.Whole-plant nitrogen-and water-relations traits,and their associated trade-offs,in adjacent muskeg and upland boreal spruce species[J].Oecologia,1997,110(2):160-168.
[27]Zhao D L,Reddy K R,Kakani V G,et al.Nitrogen deficiency effects on plant growth,leaf photosynthesis,and hyperspectral reflectance properties of Sorghum[J].European Journal of Agronomy,2005,22(4):391-403.
[28]吴福忠,包维楷,吴宁.外源施N对干旱河谷白刺花（Sophora davidii）幼苗生长,生物量及C、N、P积累与分配的影响[J].生态学报,2008,28(8):3817-3824.
[29]Yin C Y,Wang X,Duan B L,et al.Early growth,dry matter allocation and water use efficiency of two sympatric Populus species as affected by water stress[J].Environmental and Experimental Botany,2005,53(3):315-322.
[30]Villagra P E,Cavagnaro J B.Water stress effects on the seedling growth of Prosopis argentine and Prosopis alpataco[J].Journal of Arid Environments,2006,64(3):390-400.
[31]Hattenschwiler S,Korner C.Biomass allocation and canopy development in spruce model ecosystems under elevated CO2 and increased N deposition[J].Oecologia,1998,113(1):104-114.
[32]Throop H L.Nitrogen deposition and herbivory affect biomass production and allocation in an annual plant[J].Oikos,2005,111(1):91-100.
[33]Lajtha K,Schlesinger W H.Plant response to variations in nitrogen availability in a desert shrubland community[J].Biogeochemistry,1986,2(1):29-37.
[34]Wu F Z,Bao W K,Li F L,et al.Effects of drought stress and N supply on the growth,biomass partitioning and water-use efficiency of Sophora davidii seedlings [J].Environmental and Experimental Botany,2008,63(1-3):248-255.
[35]宋维民,周海燕,贾荣亮,等.土壤逐渐干旱对4种荒漠植物光合作用和海藻糖含量的影响[J].中国沙漠,2008,28(3):449-454.
[36]Warren C R,Livingston N J,Turpin D H.Photosynthetic responses and N allocation in Douglas-fir needles following a brief pulse of nutrients[J].Tree Physiology,2004,24(6):601-608.
[37]李德军,莫江明,方运霆,等.模拟氮沉降对3种南亚热带树苗生长和光合作用的影响[J].生态学报,2004,24(5):876-882.
[38]蒋高明,常杰,高玉葆,等.植物生理生态学[M].北京:高等教育出版社,2004.
[39]许大全.光合作用效率[M].上海:上海科学技术出版社,2006.
[40]李芸瑛,窦新永,彭长连.3种濒危木兰植物幼树光合特性对高温的响应 [J].生态学报,2008,28(8):3789-3797.
[41]樊卫国,李迎春.梨属4个重要种的光合特性及水分利用率[J].西南农业学报,2006,19(6):1144～1146
[42]孙惠玲,马剑英,王绍明,等.准噶尔盆地荒漠植物碳同位素组成研究[J].中国沙漠,2007,27(6):972-976.
[43]Nakaji T,Fukami M,Dokiya Y,et al.Effects of high nitrogen load on growth,photosynthesis and nutrient status of Cryptomeria japonica and Pinus densiflora seedlings[J].Trees-Structure and Function,2001,15(8):453-461.
[44]Taiz L,Zeiger E. plant physiology.2nd ed.Sunderland ,Massachusetts,USA:Sinaure Associates Inc;1998:518.
[45]Morgan J M.Osmotic components and properties associated with genotypic differences in osmoregulation in wheat[J].Australian Journal of Plant Physiology,1992,19(1):67-76.
[46]Jang J C,Leon P,Zhou L,et al.Hexokinase as a sugar sensor in higher plants[J].Plant Cell,1997,9(1):5-19.