Influence of precipitation on soil enzyme activity in sandy grasslands

doi:10.7522/j.issn.1000-694X.2025.00069

Journal of Desert Research ›› 2025, Vol. 45 ›› Issue (4): 368-377.DOI: 10.7522/j.issn.1000-694X.2025.00069

Influence of precipitation on soil enzyme activity in sandy grasslands

Jiaqi Jing¹^,²^,⁴(), Xinping Liu¹^,²^,⁴(), Yuhui He³, Jie Feng⁵, Hongjiao Hu¹^,²^,⁴, Yuanzhi Xu¹^,²^,⁴, Yao Zhang¹^,²^,⁴

^1.State Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands /, Northwest Institute of Eco-Environment and Resources，Chinese Academy of Sciences，Lanzhou 730000，China
^2.Naiman Desertification Research Station /, Northwest Institute of Eco-Environment and Resources，Chinese Academy of Sciences，Lanzhou 730000，China
^3.Lanzhou Ecological Agriculture Experimental Research Station, Northwest Institute of Eco-Environment and Resources，Chinese Academy of Sciences，Lanzhou 730000，China
^4.University of Chinese Academy of Sciences，Beijing 100049，China
^5.Tongliao Forestry and Grassland Science Research Institute，Tongliao 028314，Inner Mongolia，China

Received:2025-04-08 Revised:2025-06-13 Online:2025-07-20 Published:2025-08-18
Contact: Xinping Liu

Abstract

Abstract:

Soil enzymes are deeply involved in the nutrient cycling process within the soil system and serve as a core link connecting plant and soil nutrients. The activity of soil extracellular enzymes is extremely sensitive to changes in precipitation patterns， especially in water-scarce and nutrient-poor sandy grassland ecosystems. This study was carried out in semi-arid sandy grasslands to investigate the changes in soil extracellular enzyme activities under different precipitation conditions. The study determined the dynamic variation patterns of soil extracellular enzyme activities in the sandy grassland ecosystem and revealed the response mechanisms of soil carbon， nitrogen， and phosphorus contents as well as enzyme activities in sandy grasslands to short-term precipitation changes. The results show that：（1） Under the condition of 30% reduced precipitation， plant above-ground biomass was significantly lower than that under natural conditions （P<0.05）， while soil bulk density and electrical conductivity were significantly higher than those under natural conditions （P<0.05）；（2） Compared with the control group， the increase of 60% precipitation significantly enhanced the limitation of microbial carbon and nitrogen （P<0.05）；（3） Soil extracellular enzyme activity was highly responsive to changes in water availability. Under the condition of 60% increased precipitation， the activity of cellobiohydrolase increased to 2.47 times that of the control group， and the corresponding carbon-related enzyme activity increased by 19.7%. In contrast， nitrogen-related enzyme activity was optimal in the control group.

Key words: precipitation change, soil enzyme activity, sandy grassland, plant biomass, physical and chemical properties of soil

CLC Number:

Jiaqi Jing, Xinping Liu, Yuhui He, Jie Feng, Hongjiao Hu, Yuanzhi Xu, Yao Zhang. Influence of precipitation on soil enzyme activity in sandy grasslands[J]. Journal of Desert Research, 2025, 45(4): 368-377.

Figures/Tables 7

References 45

[1]	王明明，刘新平，何玉惠，等.科尔沁沙质草地生物量积累过程对降水变化的响应模拟［J］.生态学报，2020，40（11）：3656-3665.
[2]	Hyun S H， Yeh S W.Characteristics of internal variability on summer rainfall in Northeast Asia in a changing climate［J］.Climate Dynamics，2020，54（1）：1179-1195.
[3]	朱艳欣，桑燕芳.青藏高原降水季节分配的空间变化特征［J］.地理科学进展，2018，37（11）：1533-1544.
[4]	Shortridge J.Observed trends in daily rainfall variability result in more severe climate change impacts to agriculture［J］.Climatic Change，2019，157（3）：429-444.
[5]	周欣扬，王誉陶，李建平.黄土高原典型草原植物群落组成对降水变化的响应［J］.生物多样性，2023，31（3）：46-55.
[6]	Miranda J D， Armas C， Padilla F M，et al.Climatic change and rainfall patterns： effects on semi-arid plant communities of the Iberian Southeast［J］.Journal of Arid Environments，2011，75（12）：1302-1309.
[7]	张腊梅，刘新平，赵学勇，等.科尔沁固定沙地植被特征对降雨变化的响应［J］.生态学报，2014，34（10）：2737-2745.
[8]	王怀海，黄文达，何远政，等.短期增温和降水减少对沙质草地土壤微生物量碳氮和酶活性的影响［J］.中国沙漠，2022，42（3）：274-281.
[9]	Hopkins D W， Sparrow A D， Shillam L L，et al.Enzymatic activities and microbial communities in an Antarctic dry valley soil：responses to C and N supplementation［J］.Soil Biology and Biochemistry，2008，40（9）：2130-2136.
[10]	Chakraborty A， Zádrapová D， Dvořák J，et al.Impact of 30 years precipitation regime differences on forest soil physiology and microbial assemblages［J］.Frontiers in Forests and Global Change，2023，6：1142979.
[11]	Yanhua Z.Research advance of the relationship between soil enzyme activity and soil fertility［J］.Journal of Anhui Agricultural Sciences，2007，35（34）：11139.
[12]	Huang L， Zhang Z. Effect of rainfall pulses on plant growth and transpiration of two xerophytic shrubs in a revegetated desert area：Tengger Desert，China［J］.Catena，2016，137：269-276.
[13]	张孝良，车荣晓，段兴武，等.土壤胞外酶活性对气候变化响应的研究进展［J］.浙江农林大学学报，2023，40（4）：910-920.
[14]	倪梦颖，张秋芳，高金涛，等.亚热带杉木人工林土壤胞外酶活性对隔离降雨的季节响应［J］.生态学报，2018，38（6）：2119-2127.
[15]	Bell C W， Acosta-Martinez V， McIntyre N E，et al.Linking microbial community structure and function to seasonal differences in soil moisture and temperature in a Chihuahuan Desert grassland［J］.Microbial Ecology，2009，58：827-842.
[16]	谢志煌，高志颖，郭丽丽，等.土壤微生物活性和生物量对干湿交替的响应［J］.土壤与作物，2020，9（4）：348-354.
[17]	Kivlin S N， Treseder K K.Soil extracellular enzyme activities correspond with abiotic factors more than fungal community composition［J］.Biogeochemistry，2014，117：23-37.
[18]	朱湾湾，王攀，许艺馨，等.降水量变化与氮添加下荒漠草原土壤酶活性及其影响因素［J］.植物生态学报，2021，45（3）：309-320.
[19]	Ladwig L M， Sinsabaugh R L， Collins S L，et al.Soil enzyme responses to varying rainfall regimes in Chihuahuan Desert soils［J］.Ecosphere，2015，6（3）：1-10.
[20]	万忠梅，宋长春，郭跃东，等.毛苔草湿地土壤酶活性及活性有机碳组分对水分梯度的响应［J］.生态学报，2008，28（12）：5980-5986.
[21]	Henry H A L， Juarez J D， Field C B，et al.Interactive effects of elevated CO₂，N deposition and climate change on extracellular enzyme activity and soil density fractionation in a California annual grassland［J］.Global Change Biology，2005，11（10）：1808-1815.
[22]	闫钟清，齐玉春，李素俭，等.降水和氮沉降增加对草地土壤微生物与酶活性的影响研究进展［J］.微生物学通报，2017，44（6）：1481-1490.
[23]	Ullah M R， Carrillo Y， Dijkstra F A.Drought-induced and seasonal variation in carbon use efficiency is associated with fungi：bacteria ratio and enzyme production in a grassland ecosystem［J］.Soil Biology and Biochemistry，2021，155：108159.
[24]	王永芳，张继权，马齐云，等.21世纪初科尔沁沙地沙漠化对区域气候变化的响应［J］.农业工程学报，2016，32（）：177-185.
[25]	左小安，赵学勇，赵哈林，等.科尔沁沙质草地群落物种多样性、生产力与土壤特性的关系［J］.环境科学，2007，28（5）：945-951.
[26]	马亮乾，王耀，陈一民，等.土壤微生物和酶活性对冻融循环的响应［J］.土壤与作物，2024，13（2）：247-254.
[27]	刘新平，何玉惠，赵学勇，等.科尔沁沙地奈曼地区降水变化特征分析［J］.水土保持研究，2011，18（2）：155-158.
[28]	刘新平，赵学勇，何玉惠，等.一种野外增减雨试验装置：CN202535823U［P］.2012-11-21.
[29]	张钦，姚单君，廖恒，等.不同绿肥作物周年搭配种植下土壤微生物养分限制与土壤质量指数［J］.植物营养与肥料学报，2025，31（1）：77-88.
[30]	陈敏玲，张兵伟，任婷婷，等.内蒙古半干旱草原土壤水分对降水格局变化的响应［J］.植物生态学报，2016，40（7）：658-668.
[31]	赵莹，蔡立新，靳雨婷，等.暖干化加剧东北半干旱地区油松人工林径向生长的水分限制［J］.应用生态学报，2021，32（10）：3459-3467.
[32]	Chen X， Duan Z.Impacts of soil crusts on soil physicochemical characteristics in different rainfall zones of the arid and semi-arid desert regions of Northern China［J］.Environmental Earth Sciences，2015，3：3335-3347.
[33]	Zhang L， Xie Z， Zhao R，et al.Plant，microbial community and soil property responses to an experimental precipitation gradient in a desert grassland［J］.Applied Soil Ecology，2018，127：87-95.
[34]	朱晓亚，李子豪，林启美，等.模拟不同春季降雨量下典型草原土壤微生物磷周转特征［J］.生态学报，2020，40（8）：2655-2661.
[35]	张雨瑶，李世友.土壤磷与植物关系研究进展［J］.世界林业研究，2013，26（5）：19-24.
[36]	Bell C W， Tissue D T， Loik M E，et al.Soil microbial and nutrient responses to 7 years of seasonally altered precipitation in a Chihuahuan Desert grassland［J］.Global Change Biology，2014，20（5）：1657-1673.
[37]	Wang X， Li Y， Wang L，et al.Soil extracellular enzyme stoichiometry reflects microbial metabolic limitations in different desert types of Northwestern China［J］.Science of the Total Environment，2023，874：162504.
[38]	Liao J， Dou Y， Wang B，et al.Soil stoichiometric imbalances constrain microbial-driven C and N dynamics in grassland［J］.Science of the Total Environment，2024，924：171655.
[39]	Yu Y， Ru J， Lei B，et al.Distinct response patterns of soil micro-eukaryotic communities to early-season and late-season precipitation in a semiarid grassland［J］.Soil Biology and Biochemistry，2024，194：109427.
[40]	姚博，陈云，曹雯婕，等.呼伦贝尔退化沙地植被-土壤碳氮磷互馈关系及微生物驱动机制［J］.植物生态学报，2025，49（1）：59-73.
[41]	Henry H A L.Reprint of “Soil extracellular enzyme dynamics in a changing climate”［J］.Soil Biology and Biochemistry，2013，56：53-59.
[42]	李世清，任书杰，李生秀.土壤微生物体氮的季节性变化及其与土壤水分和温度的关系［J］.植物营养与肥料学报，2004，10（1）：18-23.
[43]	Zhou X， Zhang Y.Season and nitrogen effects on activities of three hydrolytic enzymes in soils of the Gurbantunggut Desert，Northwest China［J］.Communications in Soil Science and Plant Analysis，2014，45（12）：1699-1713.
[44]	吴文超，岳平，崔晓庆，等.古尔班通古特沙漠土壤微生物碳氮对环境因子的响应［J］.干旱区研究，2018，35（3）：515-523.
[45]	Dick R P.Soil enzyme activities as indicators of soil quality［M］//Doran J W，Coleman D C，Bezdicek D F，et al.Defining Soil Quality for A Sustainable Environment Madison，USA：Soil Science Society of America，1994：107-124.

土壤理化指标	处理
土壤理化指标	P+60%	P+30%	CK	P-30%	P-60%
容重BD/(g·cm⁻¹)	1.533±0.026^ab	1.569±0.018^ab	1.503±0.020^a	1.587±0.017^b	1.540±0.010^ab
酸碱度pH值	7.276±0.072^a	7.208±0.091^a	7.146±0.030^a	7.214±0.051^a	7.340±0.089^a
电导率EC/(S·m⁻¹)	28.20±0.819^a	27.16±1.951^a	27.00±0.804^a	36.60±2.888^b	33.22±2.659^ab
全碳TC/(g·kg⁻¹)	0.276±0.043^a	0.191±0.034^a	0.249±0.047^a	0.295±0.049^a	0.258±0.039^a
全氮TN/(g·kg⁻¹)	0.033±0.008^a	0.025±0.004^a	0.032±0.005^a	0.030±0.003^a	0.037±0.009^a
全磷TP/(g·kg⁻¹)	0.024±0.001^a	0.023±0.001^a	0.025±0.003^a	0.026±0.003^a	0.026±0.003^a
土壤碳氮比C∶N	13.199±5.563^a	7.920±1.740^a	7.685±0.860^a	10.342±1.718^a	8.555±1.671^a
土壤碳磷比C∶P	11.416±1.607^a	8.135±1.290^a	10.738±2.683^a	11.273±1.478^a	9.951±1.607^a
土壤氮磷比N∶P	1.362±0.343^a	1.080±0.122^a	1.382±0.319^a	1.197±0.212^a	1.379±0.366^a

土壤理化指标	处理
土壤理化指标	P+60%	P+30%	CK	P-30%	P-60%
容重BD/(g·cm⁻¹)	1.533±0.026^ab	1.569±0.018^ab	1.503±0.020^a	1.587±0.017^b	1.540±0.010^ab
酸碱度pH值	7.276±0.072^a	7.208±0.091^a	7.146±0.030^a	7.214±0.051^a	7.340±0.089^a
电导率EC/(S·m⁻¹)	28.20±0.819^a	27.16±1.951^a	27.00±0.804^a	36.60±2.888^b	33.22±2.659^ab
全碳TC/(g·kg⁻¹)	0.276±0.043^a	0.191±0.034^a	0.249±0.047^a	0.295±0.049^a	0.258±0.039^a
全氮TN/(g·kg⁻¹)	0.033±0.008^a	0.025±0.004^a	0.032±0.005^a	0.030±0.003^a	0.037±0.009^a
全磷TP/(g·kg⁻¹)	0.024±0.001^a	0.023±0.001^a	0.025±0.003^a	0.026±0.003^a	0.026±0.003^a
土壤碳氮比C∶N	13.199±5.563^a	7.920±1.740^a	7.685±0.860^a	10.342±1.718^a	8.555±1.671^a
土壤碳磷比C∶P	11.416±1.607^a	8.135±1.290^a	10.738±2.683^a	11.273±1.478^a	9.951±1.607^a
土壤氮磷比N∶P	1.362±0.343^a	1.080±0.122^a	1.382±0.319^a	1.197±0.212^a	1.379±0.366^a

Influence of precipitation on soil enzyme activity in sandy grasslands

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