Effect of NO on Cold Resistance of Several Wild Kentucky Bluegrass Germplasm Materials

doi:10.7522/j.issn.1000-694X.2017.00029

JOURNAL OF DESERT RESEARCH ›› 2017, Vol. 37 ›› Issue (6): 1171-1179.DOI: 10.7522/j.issn.1000-694X.2017.00029

Previous Articles Next Articles

Effect of NO on Cold Resistance of Several Wild Kentucky Bluegrass Germplasm Materials

Chen Runjuan, Lei Yawei, Bai Xiaoming, Tian Yanfeng

College of Grassland Science/Key Laboratory of Grassland Ecosystem of Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U. S. Centers for Grazingland Ecosystem Sustainability, Gansu Agricultural University Lanzhou 730070, China

Received:2017-01-13 Revised:2017-03-31 Online:2017-11-20 Published:2017-11-20

Abstract

Abstract: In order to explore the physiological mechanism of exogenous NO to improve the cold resistance of Poa effects of exogenous nitric oxide (NO) donor sodium nitroprusside (SNP) on growth, osmotic adjustment and antioxidant system of seedlings of Poa under low temperature stress which was simulated by artificial turf on wild Poa pratensis and Poa sphondylodes were studied. Results showed that with the SNP concentration increasing, the aboveground biomass, growth rate, the content of soluble sugar (SS) and soluble protein (SP), the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of the two tested materials first increased and then decreased in the concentration range of sodium nitroprusside which was 0-700 mol·L^-1 under the low temperature stress. The relative membrane permeability, the content of malondialdehyde (MDA) and free proline (Pro) trended conversely. It was harmful in high concentration of NO while low concentration of NO had a protective effect. Among all the NO experimental treatments, 100 μmol·^-1 showed the best alleviation effect on cold-resistance, the lowest level of membrane lipid peroxidation, accumulated the greatest amount of osmotic regulation substances, and the highest activity of active-oxygen-scavenging enzyme. The alleviation of exogenous NO in P. sphondylodes Trin. was more sensitive than that of Poa pratensis. under low temperature stress.

Key words: Poa pratensis, Poa sphondylodes, NO, cold resistance, osmotic regulation, protective enzymes

CLC Number:

Q945.79

Chen Runjuan, Lei Yawei, Bai Xiaoming, Tian Yanfeng. Effect of NO on Cold Resistance of Several Wild Kentucky Bluegrass Germplasm Materials[J]. JOURNAL OF DESERT RESEARCH, 2017, 37(6): 1171-1179.

References

[1] Delledonne M,Xia Y,Dixon R A,et al.Nitric oxide functions as a signal in plant disease resistance[J].Nature,1998,394:585-588.
[2] Dumer J,Klessig D F.Nitric oxide as a signal in plants[J].Current Opinion Plant Biology,1999,2:369-374.
[3] 陈银萍,王晓梅,杨宗娟,等.NO对低温胁迫下玉米种子萌发及幼苗生理特性的影响[J].农业环境科学学报,2012,31(2):270-277.
[4] Zhang W L,Shen W B,Xu L L.Effect of nitric oxide on the signal molecularity[J].Chemistry of Life,2002,22:61-62.
[5] 刘建新,胡浩斌,王鑫.外源一氧化氮供体对镉胁迫下黑麦草幼苗活性氧代谢、光合作用和叶黄素循环的影响[J].环境科学学报,2009,29(3):626-633.
[6] 俞玲,马晖玲.甘肃几种早熟禾内源激素水平及干旱适应性[J].中国沙漠,2015,35(1):182-188.
[7] 池春玉,丁国华,连永权,等.低温胁迫对三种冷季型草坪草脯氨酸含量及膜透性的影响[J].中国农学通报,2007,23(1):101-104.
[8] Lamattina L,Beligni M V,Garcia M C.Method of Enhancing the Metabolic Function and the Growing Conditi-ons of Plants and Seeds:United States,US6242384B1[P].2001.
[9] Liu X,Wang L,Liu L,et al.Alleviating effect of exogenous nitric oxide in cucumber seedling against chilling stress[J].African Journal of Biotechnology,2011,10(21):4380-4386.
[10] 张华,沈文飚,徐朗莱.一氧化氮对渗透胁迫下小麦种子萌发及其活性氧代谢的影响[J].Acta Botanica Sinica,2003,45(8):901-905.
[11] 黄忠勤,杨峰,樊继德,等.一氧化氮对汞胁迫下小麦种子萌发及抗氧化代谢的影响[J].安徽农业科学,2011,39(9):5161-5163.
[12] 阮海华,沈文飚,叶茂炳,等.一氧化氮对盐胁迫下小麦叶片氧化损伤的保护效应[J].科学通报,2001,46(23):1993-1997.
[13] 孟艳艳,范术丽,宋美珍,等.NO对生长发育中棉花叶片NO含量及其对抗氧化物酶的影响[J].作物学报,2011,37(10):1828-1836.
[14] 肖强,陈娟,吴飞华,等.外源NO供体硝普钠(SNP)对盐胁迫下水稻幼苗中叶绿素和游离脯氨酸含量以及抗氧化酶的影响[J].作物学报,2008,34(10):1849-1853.
[15] 朱涵毅,陈益军,劳佳丽,等.外源NO对镉胁迫下水稻幼苗抗氧化系统和微量元素积累的影响[J].生态学报,2013,33(2):603-609.
[16] 刘柿良,潘远智,杨容孑,等.外源一氧化氮对镉胁迫下长春花质膜过氧化、ATPase及矿质营养吸收的影响[J].植物营养与肥料学报,2014,20(2):445-458.
[17] 王光辉,李江,郑岩,等.外源CO及NO对干旱胁迫下水稻糊粉层细胞死亡的影响[J].西北植物学报,2014,34(1):13-20.
[18] 周万海,冯瑞章,师尚礼,等.NO对盐胁迫下苜蓿根系生长抑制及氧化损伤的缓解效应[J].生态学报,2015,35(11):3606-3614.
[19] 马向丽,魏小红,龙瑞军,等.外源一氧化氮提高一年生黑麦草抗冷性机制[J].生态学报,2005,25(6):1269-1274.
[20] 梁慧敏,夏阳,杜峰,等.低温胁迫对草地早熟禾抗性生理生化指标的影响[J].草地学报,2001,9(4):283-286.
[21] 邹琦.植物生理学试验指导[M].北京:中国农业出版社,2000.
[22] Urbanek H,Kuzniakgebarowska E.Elicitation of defence responses in bean leaves by Botrytis cinerea polygalacturonase[J].Acta Physiologiae Plantarum,1991,13:20-28.
[23] 杨美森,王雅芳,干秀霞,等.外源一氧化氮对冷害胁迫下棉花幼苗生长、抗氧化系统和光合特性的影响[J].中国农业科学,2012,45(15):3058-3067.
[24] 樊怀福,杜长霞,朱祝军.外源NO对低温胁迫下黄瓜幼苗生长、叶片膜脂过氧化和光合作用的影响[J].浙江农业学报,2011,23(3):538-542.
[25] 黄治远,李隆华,张云贵,等.龙眼叶片可溶性糖含量与耐寒性的关系[J].西南园艺,2004,32(4):18-19.
[26] Jian L C.Advances of the studies on the mechanism of plant cold hardiness[J].Chinese Bulletin of Botany,1992,12(3):17-22.
[27] 汤红玲,李江,陈惠萍.外源一氧化氮对香蕉幼苗抗冷性的影响[J].西北植物学报,2010,30(10):2028-2033.
[28] 吴雁斌,王一航,张武,等.PEG与SNP对低温下马铃薯试管苗相关生理指标的影响[J].核农学报,2010,24(3):645-649.
[29] 吴锦程,陈伟建,蔡丽琴,等.外源NO对低温胁迫下枇杷幼果抗氧化能力的影响[J].林业科学,2010,46(9):73-78.
[30] 王芳,常盼盼,陈永平,等.外源NO对镉胁迫下玉米幼苗生长和生理特性的影响[J].草业学报,2013,22(2):178-186.
[31] Beligni M V,Lamattina L.Nitric oxide counteracts cytotoxic processes mediated by reactive oxygen species in plant tissues[J].Planta,1999,208(3):337-344.
[32] 王罗霞,赵志光,王锁民.一氧化氮对水分胁迫下小麦叶片活性氧代谢及膜脂过氧化的影响[J].草业学报,2006,15(4):104-108.
[33] 张艳艳,刘俊,刘友良.一氧化氮缓解盐胁迫对玉米生长的抑制作用[J].植物生理与分子生物学学报,2004,30(4):455-459.

Effect of NO on Cold Resistance of Several Wild Kentucky Bluegrass Germplasm Materials

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Jinxia Li, Yanan Zhu, Xiaomei Sun, Guojun Han, Nianlai Chen. Effects of nitrogen and phosphorus addition on non-structural carbohydrate properties of vegetative organs in Lycium ruthenicum [J]. Journal of Desert Research, 2021, 41(2): 200-211.
[2]	Shengxia Wang, Fei Wang. Response of oasis area to the surface runoff in Hexi inland river basin of China [J]. Journal of Desert Research, 2021, 41(2): 231-241.
[3]	Mengzhu Liu, Yanfang Wang, Hongwei Pei. The changes of land use and carbon storage in the northern farming-pastoral ecotone under the background of returning farmland to forest （grass） [J]. Journal of Desert Research, 2021, 41(1): 174-182.
[4]	Mingzhu Zhao, Ruiping Zu, Junzhan Wang, Lihai Tan. Grain size characteristics of sediment along the Hami-Lop Nor Railway [J]. Journal of Desert Research, 2021, 41(1): 19-27.
[5]	Yang Zhao, Yanxia Pan, Jieqiong Su, Zhishan Zhang. Research status and development trend of green and environmental protection technologies on desertification land prevention in arid region of China [J]. Journal of Desert Research, 2021, 41(1): 195-202.
[6]	Yunhua Ma, Tu Feng, Yangzheng Li, Qingqing Jin. Adaptability of the Hippophae rhamnoides seedlings on different degrees of soil dressing for rocky desertification [J]. Journal of Desert Research, 2021, 41(1): 228-233.
[7]	Fangming Zeng, Hongpan Xue. Elemental compositions of the late Quaternary loess-paleosol on the northeastern Qinghai-Tibet Plateau and their implications for provenance [J]. Journal of Desert Research, 2020, 40(6): 105-117.
[8]	Xiaoming Bai, Yongmei Zhang, Hui Chen, Junlan Zhang. Soil nutrient status of Poa pratensis distribution areas in Gansu， China [J]. Journal of Desert Research, 2020, 40(6): 242-249.
[9]	Jiantao Zhang, Minzhong Wang, Qing He, Honglin Pan, Lu Meng, Yanhui Wang. Variation characteristics of nocturnal low-level jet in summer over the hinterland of Taklimakan Desert [J]. Journal of Desert Research, 2020, 40(5): 89-100.
[10]	Caixia Zhang, Junpeng Lou, Diwen Cai. Major and minor elements content for fifteen-five vegetation samples in desert areas [J]. Journal of Desert Research, 2020, 40(4): 18-23.
[11]	Ning Baoying, Ma Jianxia, Jiang Zhide. Influencing factors and conceptual model for suitability evaluation of ecological control technologies: an example in desertification control [J]. Journal of Desert Research, 2020, 40(2): 9-16.
[12]	Li Xiang, Su Zhizhu, Ma Yijuan, Zhang Caixia, Liu Miaomiao. Holocene climatic instability record in the southeastern margin of Mu Us Desert [J]. Journal of Desert Research, 2020, 40(2): 109-117.
[13]	Zhao Zefang, Wei Haiyan, Guo Yanlong, Luan Wenfei, Zhao Zebin. Impact of climate change on the suitable habitatdistribution of Gymnocarpos przewalskii, a relict plant [J]. Journal of Desert Research, 2020, 40(2): 125-133.
[14]	Sha Jie, Chen Yuan, Guo Fengxia, Hu Longsheng, Liang Wei, Zhao Ruiming. Effects of exogenous Ca²⁺ on re-greening physiological characteristics of Lamiophlomis rotata [J]. Journal of Desert Research, 2020, 40(2): 156-164.
[15]	Jia Peiyu, Chen Jianing. The spatial and temporal changes of ecology-resources-economy coordinated development in ecologically fragile area of Shanxi, China [J]. Journal of Desert Research, 2020, 40(1): 179-186.