干旱胁迫下牛心朴子的渗透调节机制研究

摘要/Abstract

摘要： 为探讨荒漠植物牛心朴子(Cynanchum komarovii)响应干旱胁迫的渗透调节机制,采用不同浓度（0~25%）的聚乙二醇（PEG-6000）对愈伤组织进行模拟干旱胁迫,检测了不同程度干旱胁迫下牛心朴子中渗透调节物质K+、Na+、脯氨酸、甜菜碱和可溶性糖（果糖、蔗糖和海藻糖）的含量。结果显示,与对照组相比,处理组牛心朴子的脯氨酸和可溶性糖含量显著升高（P<0.05）,且具有浓度和时间依赖效应。在可溶性糖中,果糖和蔗糖只在高浓度处理组有所增加,而海藻糖含量在所有处理组均明显升高（P<0.05）,且增幅较大（10%PEG浓度组为31%,25%PEG浓度组为98%）,同时海藻糖含量与PEG处理浓度的相关系数最大（R2=0.9437,P<0.01）。以上研究结果表明,海藻糖是牛心朴子响应干旱胁迫主要的渗透调节物质。

关键词: 牛心朴子, 渗透调节, 海藻糖, 果糖, 蔗糖

Abstract: Cynanchum komarovii is one of the desert plants with drought resistance. To study its osmotic adjustment mechanism under drought stress, the calluses of Cynanchum Komarovii were treated with PEG-6000 at different concentration (0~25%), and then content of the osmotic adjustment substances (K+, Na+, proline, betaine, soluble sugars and so on) were measured. Results showed that with the rise of PEG-6000 concentration, the contents of proline and soluble sugars increased obviously (P<0.05). Under drought stress, the contents of fructose and sucrose increased at high concentration PEG-6000 treatments, while the content of trehalose increased very obviously at all PEG-6000 treatments, with 31% enhancement at 10% PEG-6000 treatment and 98% at 25% PEG-6000 treatment, respectively. The correlation analysis showed that the coefficient between trehalose content and PEG-6000 concentration was the highest with R2=0.9437 (P<0.01). These results suggest that trehalose is the primary osmotic adjustment substance of Cynanchum komarovii resisting to drought stress.

Key words: Cynanchum komarovii, osmotic adjustment, trehalose, fructose, sucrose

中图分类号:

Q945

陈翠云, 赵昕, 李新荣. 干旱胁迫下牛心朴子的渗透调节机制研究[J]. 中国沙漠, 2012, 32(5): 1275-1282.

CHEN Cui-yun, ZHAO Xin, LI Xin-rong. Osmotic Adjustment Mechanism of Cynanchum komarovii under Drought Stress[J]. JOURNAL OF DESERT RESEARCH, 2012, 32(5): 1275-1282.

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