红砂愈伤组织适应盐胁迫的渗透调节机制研究

摘要/Abstract

摘要： 测定在不同盐胁迫下红砂愈伤组织中几种渗透调节物质的累积量,研究盐胁迫下红砂的渗透调节机制。研究结果表明, 在盐胁迫下,红砂愈伤组织的相对生长率随着盐浓度的增加先升高后降低。红砂愈伤组织具有很强的耐盐能力,低于100 mM NaCl处理可促进红砂的生长,大于100 mM NaCl处理,则会抑制红砂生长。红砂愈伤组织中脯氨酸、甘氨酸甜菜碱、类黄酮和海藻糖在盐胁迫下大量累积,是红砂渗透调节能力改善的积极反应,也是红砂耐盐性强的体现和重要原因。

关键词: 红砂, 愈伤组织, 盐胁迫, 渗透调节

Abstract: Reaumuria soongorica (Pall.) Maxim., a perennial semi-shrub, is widely found in semi-arid areas in northwestern China. It is a resurrection plant that can tolerate prolonged protoplast desiccation conditions and revive upon rehydration. The present study aimed to investigate the salt tolerance mechanisms of calluses induced from R. soongorica. The growth and organic osmolytes under salt stress were examined. Results were summarized as follows. The relative growth rate of R. soongorica callus reached the maximum in 14 days presence of 100 mM NaCl and it was inhibited with further increasing of NaCl concentrations. The callus had higher relative growth rate in low salt concentrations than in high NaCl concentrations or no NaCl, which indicated that R. soongorica callus need salt in the progress of R. soongorica growing. Examination of the osmotic substances under salt stress showed accumulation of proline, glycine betaine and trehalose with increasing of NaCl concentrations, and the strong osmotic adjustment ability was a important factor in the salt resistant mechanism. The results indicated that the calluses of R. soongorica retained similar to other halophytes in response to salt stress.

Key words: Reaumuria soongorica, callus, salt stress, osmotic adjustment

中图分类号:

Q945

谭会娟;李新荣;赵昕;刘玉冰. 红砂愈伤组织适应盐胁迫的渗透调节机制研究[J]. 中国沙漠, 2011, 31(5): 1119-1123.

TAN Hui-juan;LI Xin-rong;ZHAO Xin;LIU Yu-bing. Study on Mechanisms of Osmo regulation of Reaumuria soongorica Callus in Adapting to Salt Stress[J]. JOURNAL OF DESERT RESEARCH, 2011, 31(5): 1119-1123.

参考文献

[1]Boyer J S.Plant productivity and environment[J].Science,1982,218:443-448.
[2]侯学煜.中国温带干旱荒漠区植被地理分布[J].植物学集刊,1987(2):37-66.
[3]张元明,潘伯荣,尹林克,等.中国柽柳科(Tamari caceae)花粉形态研究及其分类意义的探讨[J].西北植物学报,2001,21(5):857-864.
[4]张道远.柽柳科系统学研究中的一些问题探讨[J].云南植物研究,2005,27(5):471-478.
[5]刘家琼,邱明新,蒲锦春,等.我国荒漠典型超旱生植物——红砂[J].植物学报,1982,24(5):485-488.
[6]刘媖心.中国沙漠植物志(第二卷) [M].北京:科学出版社,1987:368-370.
[7]阿拉善盟农牧业区划编辑委员会.阿拉善盟农牧业区划[M].呼和浩特:内蒙古人民出版社,1992:430-452.
[8]陈拓,冯虎元,徐世建,等.荒漠植物叶片碳同位素组成及其水分利用效率[J].中国沙漠,2002,22(3):288-291.
[9]李骁,王迎春,征荣.西鄂尔多斯地区强旱生小灌木水分参数的研究[J].中国沙漠,2005,25(4):581-586.
[10]刘玉冰,张腾国,安黎哲,等.红砂正常和脱水组织中总RNA提取的改进CTAB法[J].中国沙漠,2006,26(4):600-603.
[11]刘玉冰,张腾国,李新荣,等.红砂(Reaumuria soongorica)忍耐极度干旱的保护机制:叶片脱落和茎中蔗糖累积[J].中国科学(C辑),2006,36(4):328-333.
[12]刘玉冰,赵昕,谭会娟.荒漠植物红砂愈伤组织诱导与增殖研究[J].中国沙漠,2008,28(2):255-257.
[13]李新荣,石庆辉,张景光,等.沙坡头地区人工植被演变过程中植物多样性变化的研究[J].中国沙漠,1998,18(增):23-29.
[14]中国科学院内蒙古-宁夏综合考察队.内蒙古植被[M].北京:科学出版社,1985.
[15]张殿忠,汪沛洪,赵会贤.测定小麦叶片游离脯氨酸含量的方法[J].植物生理学通讯,1990(4):62-65.
[16]Dong Y S,Yang Q Z,Jia S R,et al.Effects of high pressure on the accumulation of trehalose and glutathione in the Saccharomyces cerevisiae cells[J].Biochemical Engineering Journal,2007,37(2):226-230.
[17]Grattan S R,Grieve C M.Mineral element acquisition and growth response of plants grown in saline environments[J].Agriculture,Ecosystems and Environment,1992,38:275-300.
[18]项昭保.吸光光度法测定荞麦秸中总黄酮[J].理化检验——化学分册,2002,38(9):436-437.
[19]Levitt J.Response of plants to environmental stress[M].New York:Academic Press,1980:365-434.
[20]张海燕,赵可夫.盐分和水分胁迫对碱蓬幼苗渗透调节效应的研究[J].植物学报,1998,1:56-61.
[21]Albert R.Salt regulation in halophytes[J].Decologia,1975,21:57-70
[22]薛炎,王迎春.盐生植物长叶红砂泌盐特性的研究[J].中国沙漠,2008,28(3):437-442.
[23]Yoshiba Y,Kiyosue T,Katagiri T,et al.Correlation between the induction of a gene for delta1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress[J].The Plant Journal,1995,7(5):751-760.
[24]Wang WX,Vinocur B,Altman A.Plant responses to drought,salinity and extreme temperatures: towards genetics engineering for stress tolerance[J].Planta,2003,218:1-14.
[25]Goddijn O J M,van Dun K.Trehalose Metabolism in Plants[J].Trends in Plant Science,1999,4(8):315-319.
[26]Goddijn O,Smeekens S.Sensing trehalose biosynthesis in plants[J].The Plant Journal,1998,14(2):143-146.
[27]封德顺.海藻糖的生物学功能简介[J].生物学通报,1999,34(2):13-14.
[28]McCue K F,Hanson A D.Drought and salt tolerance: towards under standing and application[J].Trends in Biotechnology,1990,8:358-362.
[29]Flowers T J,Hajibagheri M A,Clipson N W.Halophytes[J].The quarterly review of biology,1986,61:313-337.
[30]Rhodes D,Hanson A D.Quaternary ammonium and terriary sulfonium compounds in higher plants[J].Annual Review of Plant Physiology and Plant Molecular Biology,1993,44:357-384.
[31]Zhang F,Yang Y L,He W L,et al.Effects of salinity on growth and compatible solutes of callus induced from Populus euphratica[J].In Vitro Cellular and Developmental Biology-Plant,2004,40:491-494.
[32]Bohnert H J,Nelson D E,Jensen R G.Adaptations to environmental stresses[J].Plant Cell,1995,7:1099-1111.
[33]王淑丽,赵昕,谭会娟,等.荒漠植物花棒耐盐性的傅立叶红外光谱法研究[J].中国沙漠,2008,28(5):874-878.
[34]Quick P,Siegl G,Neuhaus E,et al.Short-term water stress leads to a stimulation of sucrose synthesis by activating sucrosephosphate synthase[J].Planta,1989,177:535-546.
[35]Wang Z,Stutte G W.The role of carbohydrates in active osmotic adjustment in apple under water stress[J].Journal of the American Society for Horticultural Science,1992,117:816-823.
[36]Basnayake J,Ludlow M M,Cooper M,et al.Genotypic variation of osmotic adjustment and desiccation to lerance in contrasting sorghum: inbred lines[J].Field Crops Research,1993,35:51-62.
[37]张有福,陈拓,费贯清,等.盐度对三种荒漠植物渗透调节物质积累影响的研究[J].中国沙漠,2007,27(5):787-790.