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中国沙漠 ›› 2008, Vol. 28 ›› Issue (1): 131-136.

• 生物土壤与生态 • 上一篇    下一篇

光周期对沙枣尺蠖病菌(Dh菌株)离体培养的影响

朱建兰;张自和;常永义   

  1. 1.甘肃农业大学草业学院, 甘肃 兰州 730070; 2.兰州大学 农业部草地农业生态系统学重点开放实验室,甘肃 兰州 730020; 3.甘肃农业大学农学院, 甘肃 兰州 730070
  • 收稿日期:2006-11-20 修回日期:2007-02-09 出版日期:2008-01-20 发布日期:2008-01-20

Effect of Photoperiod on Growth and Spore Generation of an Emericella quadriclineata Strain

ZHU Jian lan; ZHANG Zi he; CHANG Yong yi   

  1. 1.College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, China; 2.The Key Open Laboratory of Grassland Agro-Ecosystems of Agricultural Ministry, Lanzhou University, Lanzhou 730020, China; 3.College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2006-11-20 Revised:2007-02-09 Online:2008-01-20 Published:2008-01-20

摘要: 研究了光周期对沙枣尺蠖病菌(Emericella quadriclineata)Dh菌株菌丝生长及产孢量的影响。结果表明,光照对液培菌丝生物量和平板培养产孢有明显的影响,长光照有利于菌丝生长,菌丝生物量明显高于短光照和全黑暗;培养4 d菌丝生物量最大,其中以18 h光照与6 h黑暗交替菌丝生物量最大。光照对Dh菌株平板产孢具有一定的促进作用,连续光照与各温度组合的累积产孢量显著高于连续黑暗下各温度组合,以全光照25℃培养7 d产孢量最高,第3天至第5天时增长率达到顶点;30℃和35℃下无论是光照还是黑暗条件均不适合产孢,其产孢速率呈持续下降状态。紫外光对Dh菌株生长的影响程度与照射时间有关,在供试范围内,照射时间越长菌落生长越慢。

关键词: 光周期, Dh菌株, 生物量, 产孢速率

Abstract: The influence of photoperiod on the growth and spore generation of a strain of Emericella quadriclineata isolated from a cadaver of the mulberry looper, Apocheima cinerarius, was explored. Its hyphal growth was facilitated in liquid culture under long light, yielding significantly greater biomass than that under short light or in dark. The biomass peaked after 4-day incubation at the regime of Light:Dark 18∶6, an optimal photoperiod favoring spore generation on medium plates. At different temperature, sporulation capacity of the strain cultured under full light was much greater than that in full dark, peaked on day 7th at 25℃ under full light with a maximal increase rate being achieved on day 3rd to 5th. At 30℃ or 35℃, whether light or dark, the sporulation was quite poor. The effect of ultraviolet light on the growth of the strain increased with the decrease of exposure time.

Key words: photoperiod, Dh strain, biomass yield, spore generating rate

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