低温胁迫下巴旦杏的形态生理变化及抗寒性研究

摘要/Abstract

摘要： 莎车1号、英吉沙2号和莎车18号是新疆喀什地区巴旦杏的主栽品种。通过测定低温胁迫下细胞膜透性、叶绿素荧光参数的变化,对巴旦杏品种的抗寒性进行了比较研究,并进行了冻害分级。结果表明,随处理温度降低,相对电导率逐渐增加,枝条细胞膜受损程度在加重。引起巴旦杏品种低温伤害的临界温度范围为-24～-27 ℃,3个品种枝条半致死温度（LT50）分别为-24.9 ℃、-29.3 ℃、-27.7 ℃,英吉沙2号的抗寒性较强。随处理温度降低,巴旦杏枝条基础荧光（Fo）值逐渐上升,至某一温度达到峰值后开始下降,光系统（PSⅡ）最大光化学效率（Fv/Fm）值呈明显下降趋势。-24～-27 ℃左右的低温会造成PSⅡ结构的损伤,且品种抗寒性越差,PSⅡ结构受损越明显。综合分析结果表明,供试的3个巴旦杏品种中, 英吉沙2号抗寒性较强,莎车18号次之,莎车1号抗寒性较弱。

关键词: 巴旦杏, 低温胁迫, 相对电导率, 半致死温度（LT50）, 叶绿素荧光

Abstract: Shache No.1, Yingjisha No.2 and Shache No.18 are the main cultivars of Almond in Kashgar region of Xinjiang, China. The permeability of plasma membrane and chlorophyll fluorescence of the different cultivars of Almond under different low temperature stress were measured to research characteristic changes and their cold resistance with the one year old dormant branches of the cultivars as tested materials. Results showed that the relative conductivity increased, and the damage to plasma membrane increased with decrease of temperature. The critical freezing temperature resulting in damage to the cultivars of Almond ranged from -24 ℃ to -27 ℃. The semilethal temperature of Shache No.1, Yingjisha No.2 and Shache No.18 were -24.9 ℃, -29.3 ℃, -27.7 ℃, respectively. The minimum fluorescence (Fo) of Almond increased firstly with decrease of temperature, and then decreased after reaching a peak value at typical temperature. The maximum photochemical efficiency (Fv/Fm) decreased evidently with decrease of temperature. The damage to photosystem Ⅱ (PSⅡ) could be induced by low temperature of -24～-27 ℃, and the weaker the cold resistance was, the obvious the damage was. Comprehensive analysis to these three cultivars of almond showed that Yingjisha No.2 had the strongest cold resistance, followed by Shache No.18, and the cold resistance of Shache No.1 was the worst.

Key words: Almond, low temperature stress, relative conductivity, semilethal temperature, chlorophyll fluorescence

中图分类号:

Q945.7

吉春容, 邹陈, 陈丛敏, 刘立宏, 李新建. 低温胁迫下巴旦杏的形态生理变化及抗寒性研究[J]. 中国沙漠, 2012, 32(4): 955-962.

JI Chun-rong, ZOU Chen, CHEN Cong-min, LIU Li-hong, LI Xin-jian. Morphological and Physiological Characteristics of Almond under Low Temperature Stress and Its Cold Resistance[J]. JOURNAL OF DESERT RESEARCH, 2012, 32(4): 955-962.

参考文献

[1]曹琴,孔维府,温鹏飞.植物耐寒及其基因表达研究进展[J].生态学报,2004,24(4):806-811.

[2]Kreyling J,Beierkuhnlein C,Jentsch A.Effects of soil freeze-thaw cycles differ between experimental plant communities[J].Basic and Applied Ecology,2009,10:1016-1027.

[3]何维勋,冯玉香,夏满强.解冻速率对作物霜冻害的影响[J].应用气象学报,1993,4(4):440-445.

[4]崔建玲,邓小勤,李万福,等.巴旦杏防冻技术[J].山西果树,2010,5:43-44.

[5]古再丽努尔·沙吾提.巴旦杏受冻后的恢复措施[J].农村科技,2008,12:42-43.

[6]魏小亚,马忠萍,刘春容.裕民县野生巴旦杏种植的气候条件分析[J].现代农业科技,2009,20:301-303.

[7]何开跃,李晓储,黄利斌,等.3种含笑耐寒生理机制研究[J].南京林业大学学报(自然科学版),2004,28(4):62-64.

[8]Beck E,Heim R,Hansen J.Plant resistance to cold stress:Mechanisms and environmental signals triggering frost hardening and dehardening[J].Journal of Bioscience,2004,29:440-459.

[9]王文举,张亚红,牛锦凤,等.电导法测定鲜食葡萄的抗寒性[J].果树学报,2007,24(1):34-37.

[10]薛建辉,苏敬,田如男,等.自然降温过程中5个常绿阔叶树种的抗寒性分析[J].植物资源与环境学报,2009,18(1):52-56.

[11]曹慧明,史作民,周晓波,等.植物对低温环境的响应及其抗寒性研究综述[J].中国农业气象,2010,31(2):310-314.

[12]朱成刚,李卫红,马晓东,等.塔里木河下游干旱胁迫下的胡杨叶绿素荧光特性研究[J].中国沙漠,2011,31(4):927-936.

[13]谢新,陈晓燕.和静县2006年冬季低温冻害的成因分析[J].新疆农业科学,2008,45(S1):269-270.

[14]邵文鹏,赵兰勇,吴殿鸣,等.用电导法配合Logistic方程鉴定桉树的抗寒性[J].山东林业科技,2009,(1):28-29.

[15]严寒静,谈锋.自我降温过程中栀子叶片膜保护系统的变化与低温半致死温度的关系[J].植物生态学报,2000,24(1):91-95.

[16]王有科,刘冰,郭凤霞.花椒品种抗冻性比较研究[J].中国沙漠,2006,26(6):1041-1045.

[17]Meng Q R,Liang Y Q,Wang W F,et al.Study on super-cooling point and freezing point in floral organs of Apricot[J].Agricultural Science in China,2007,6(11):1330-1335.

[18]谢晓金,郝日明,张纪林.常绿阔叶树种的耐低温特性及其生态学评价[J].生态学报,2004,24(11):2671-2678.

[19]张亚娟,谢忠奎,赵学勇,等.水分胁迫对东方百合光合特性、叶绿素荧光参数及干物质积累的影响[J].中国沙漠,2011,31(4):884-888.

[20]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448.

[21]陈年来,张玉鑫,安黎哲.NaCl胁迫对甜瓜叶片气体交换和叶绿素荧光特性的影响[J].中国沙漠,2009,29(6):1129-1133.

[22]艾尼瓦尔·依沙木丁.2008年和田地区冬季林果业冻害成因分析及预防措施[J].沙漠与绿洲气象,2009,3(1):49-52.

[23]谢晓金,郝日明.南京地区12种常绿阔叶树种冬季抗寒性动态变化[J].生态学报,2009,29(4):2149-2154.

[24]蔡子平,陈垣,郭凤霞,等.外源Ca2+对藏药独一味幼苗速冷冻抗性的效应[J].中国沙漠,2009,29(5):928-932.

[25]徐叶挺,李疆,罗淑萍,等.低温胁迫下野生巴旦杏抗寒生理指标的测定[J].新疆农业大学学报,2008,31(4):1-4.

[26]朱京琳.新疆巴旦杏[M].乌鲁木齐:新疆人民出版社,1984.

[27]傅玮东,喻树龙,韩勇.新疆巴旦杏与气象服务[J].新疆气象,2002,25(6):38-40.

[28]王可玢,赵福洪,王孝宜,等.用体内叶绿素荧光诱导动力学鉴定番茄的抗冷性[J].植物学通报,1996,13(2):29-33.

[29]张静,张元明.冻融过程对生物结皮中齿肋赤藓叶绿素荧光特性的影响[J].中国沙漠,2011,31(6):1479-1487.

[30]何跃君,薛立,任向荣,等.低温胁迫对六种苗木生理特性的影响[J].生态学杂志,2008,27(4):524-531.