镍、铜对矿业废弃地先锋植物盐生草和骆驼蓬抗氧化物质和渗透调节物质积累的影响

摘要/Abstract

摘要：

以盐生草(Halogeton glomeratus)和骆驼蓬(Peganum harmala)幼苗为材料,采用盆栽试验研究土壤中不同浓度(0、50、100、200、400 mg·kg-1)镍(Ni)、铜(Cu)处理对盐生草和骆驼蓬幼苗过氧化氢(H2O2)、抗坏血酸(ASA)、维生素E(VE)、谷胱甘肽(GSH)、脯氨酸和可溶性糖含量的影响。结果表明,盐生草和骆驼蓬叶片及根部过氧化氢(H2O2)含量随介质中Ni和Cu浓度的增加而增加。Ni胁迫下盐生草叶片中GSH含量随Ni浓度增加呈增加趋势,根部VE含量在高浓度Ni(≥100 mg·kg-1)胁迫下显著积累;Cu胁迫下盐生草叶片和根部GSH、ASA和VE含量较对照显著积累,三者协同作用缓解Cu胁迫诱导的氧化损伤。Ni胁迫下骆驼蓬叶片GSH和ASA含量、根部ASA含量较对照显著增加;Cu胁迫下骆驼蓬叶片中GSH和ASA含量随Cu浓度增加呈上升趋势,根部ASA含量较对照显著增加,VE在100 mg·kg-1和200 mg·kg-1的Cu处理下较对照显著增加。Ni和Cu胁迫使得盐生草和骆驼蓬幼苗脯氨酸和可溶性糖含量较对照显著积累。

关键词: 镍, 铜, 荒漠植物, 抗氧化物质, 渗透调节物质

Abstract:

A pot experiment was conducted to study the influence of different concentrations (0, 50, 100, 200, 400 mg·kg 1) of Ni and Cu in soil on the contents of hydrogen peroxide (H2O2), ascorbic acid (ASA), glutathione (GSH), vitamin E (VE), proline and soluble sugar in leaves and roots of Halogeton glomeratus and Peganum harmala seedlings. H2O2 contents in leaves and roots of the two plant species increased with increasing Ni and Cu concentrations. In leaves of H. glomeratus, GSH content increased with increasing Ni concentration, and VE accumulated significantly in roots at higher Ni concentration (≥100 mg·kg-1). In leaves and roots of H. glomeratus under Cu stress, ASA, GSH and VE contents accumulated significantly compared with no Cu treatment, suggesting they together play an important role in weakening Cu induced oxidative damage. Under Ni stress, GSH and ASA accumulated significantly in leaves of P. harmala compared with no Ni stress, while only ASA content accumulated evidently in roots of P. harmala. Under Cu stress, GSH and ASA contents increased in leaves of P. harmala with increasing Cu concentration, and ASA content accumulated significantly in roots compared with no Cu treatment, and VE content increased markedly at 100 or 200 mg·kg 1 Cu treatment. Under Ni and Cu stress, proline and soluble sugar in H. glomeratus and P. harmala seedlings accumulated significantly compared with no Ni or no Cu treatment.

Key words: nickel, copper, desert plants, antioxidants, osmotic adjustment substances

中图分类号:

Q945

鲁艳1, 李新荣2, 何明珠2, 冯丽2, 曾凡江1. 镍、铜对矿业废弃地先锋植物盐生草和骆驼蓬抗氧化物质和渗透调节物质积累的影响[J]. 中国沙漠, 2012, 32(6): 1681-1690.

LU Yan1, LI Xin-rong2, HE Ming-zhu2, FENG Li2, ZENG Fan-jiang1. Effects of Ni and Cu Treatments on Contents of Antioxidants and Osmotic Adjustment Substances in Halogeton glomeratus and Peganum harmala, Two Pioneer Plants Growing in Mining Wasteland[J]. JOURNAL OF DESERT RESEARCH, 2012, 32(6): 1681-1690.

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