Dynamic of Culturable Microorganisms in Biological Soil Crusts under Trampling Disturbance

doi:10.7522/j.issn.1000-694X.2018.00013

Journal of Desert Research ›› 2019, Vol. 39 ›› Issue (1): 119-126.DOI: 10.7522/j.issn.1000-694X.2018.00013

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Dynamic of Culturable Microorganisms in Biological Soil Crusts under Trampling Disturbance

Bao Tianli^1,2, Zhao Yunge¹, Gao Liqian³, Shi Yafang³

1. State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Sciences/Ministry of Water Resources, Yangling 712100, Shaanxi, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. College of Resources and Environment, Northwest A & F University, Yangling 712100, Shaanxi, China

Received:2017-11-28 Revised:2018-01-16 Published:2019-02-14

Abstract

Abstract: Biological soil crusts (biocrusts) exert remarkable influence on soil microorganism quantity and community structure. Disturbance is a ubiquitous natural phenomenon, which may cause changes in the coverage and composition of biocrusts and consequently affect soil microorganisms. The dynamic variation of microorganism quantity of biocrust layer and 0-2 cm soil layers in an eight-year development biocrustal soil were investigated by using plating method in order to reveal the effect of disturbance on soil microorganisms. The results showed that:(1) The quantity of bacteria, fungi and actinomyces changed on the second day since disturbance. Moreover, the fluctuation amplitude of quantity of bacteria, fungi and actinomyces in the biocrust layer were 91.2×10⁵-303.5×10⁵ cfu·g^-1, 0-487.2×10³ cfu·g^-1, and 23.0×10⁵-376.1×10⁵ cfu·g^-1, respectively. The fluctuation amplitude of quantity of bacteria, fungi and actinomyces in 0-2 cm soil layer were 65.6×10⁵-792.3×10⁵ cfu·g^-1, 8.0×10³-506.3×10³ cfu·g^-1 and 17.3×10⁵-801.3×10⁵ cfu·g^-1, respectively. (2) Bacterial quantity in biocrust layer and 0-2 cm soil layers restabilized in the tenth and seventh day after disturbance, respectively. Quantity of fungal in biocrust layer and 0-2 cm layers tended to be stable after 16 and 8 days of disturbance, respectively. Quantity of soil actinomyces in biocrust layer and 0-2 cm soil layers reached a new stabilization in eight and four days since disturbance, respectively. The stable period of soil microbial quantity lagged behind in biocrust layer than 0-2 cm soil layer. (3) Bacterial quantity decreased by 81.8% and 79.6% in biocrust layer and 0-2 cm soil layers in the new stable stage compared to that before disturbance. However, fungal quantity was 7.43 times higher in biocrust layer, and it decreased by 70.1% in 0-2 cm soil layer. Further, quantity of actinomyces decreased by 46.5% and 72.6% in biocrust layer and 0-2 cm soil layers, respectively. (4) Disturbance had a significant effect on soil microbial community structure, the proportion of different soil microorganisms became actinomyces > bacteria > fungi from bacteria > actinomyces > fungi. Results of the study suggested that disturbance can significantly affect soil microbial quantity, but the quantity of microorganisms will reach a new stable status as time goes on. Therefore, the sampling time could be an important influence factor in the study of disturbance on soil microorganisms.

Key words: biocrusts, bacteria, fungi, actinomyces, dynamic response

CLC Number:

S154.3

Bao Tianli, Zhao Yunge, Gao Liqian, Shi Yafang. Dynamic of Culturable Microorganisms in Biological Soil Crusts under Trampling Disturbance[J]. Journal of Desert Research, 2019, 39(1): 119-126.

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Dynamic of Culturable Microorganisms in Biological Soil Crusts under Trampling Disturbance

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