Soil fungal community structure and functional characteristics associated with Pinussylvestris var. mongolica plantations in the Horqin Sandy Land

doi:10.7522/j.issn.1000-694X.2023.00035

Journal of Desert Research ›› 2023, Vol. 43 ›› Issue (4): 241-251.DOI: 10.7522/j.issn.1000-694X.2023.00035

Soil fungal community structure and functional characteristics associated with Pinussylvestris var. mongolica plantations in the Horqin Sandy Land

Dandan Li¹(), Jiawen Li¹, Guanglei Gao¹^,²^,³(), Ying Zhang¹^,³, Yue Ren¹, Ye Liu¹, Peishan Zhao¹

^1.MOE Engineering Research Center of Forestry Ecological Engineering，School of Soil and Water Conservation /, Beijing Forestry University，Beijing 100083，China
^2.Yanchi Ecology Research Station of the Mu Us Sandy Land /, Beijing Forestry University，Beijing 100083，China
^3.Key Laboratory of Soil and Water Conservation of the State Forestry and Grassland Administration, Beijing Forestry University，Beijing 100083，China

Received:2023-01-16 Revised:2023-04-12 Online:2023-07-20 Published:2023-08-14
Contact: Guanglei Gao

Abstract

Abstract:

To explore a firm basis for sustainable management of Pinussylvestris var. mongolica， soil fungal community structure and functional characteristics as well as the driving soil properties were revealed in different stand ages of P. sylvestris plantations in the Horqin Sandy Land. We sampled the soil of P. sylvestris plantations with young-mature （13 a）， half-mature （22 a）， nearly-mature （34 a）， mature （41 a） and over-mature （55 a）， and soil fungal communities were identified by Illumina MiSeq high-throughput sequencing and FUNGuild platform. The results showed that：（1） In the Horqin Sandy Land， 922 fungal OTUs were obtained from soil samples of P. sylvestris plantations， and belonged to 254 genera， 98 families， 67 orders， 25 classes， and 14 phyla. Ascomycota and Basidiomycota were predominated， and the dominant genera were Calostoma and Amphinema. （2） With stand aging， the proportion of symbiotic fungi and the dominated functional groups ectomycorrhizal fungi climbed up and then declined， with a minimum in the mature plantations； the proportion of saprophytic fungi and the dominated functional groups undefined saprophytic fungi decreased after a little increase， with a maximum in mature plantations； the distribution of pathotrophic fungi was relatively uniform. （3） With stand aging， soil fungal network showed a differentiated tendency which simplified first and then complicated. The network of nearly-mature plantations was the simplest， and the over-mature plantations was the most complex followed by the mature plantations. （4） Soil organic matter， pH， alkali-hydrolyzable nitrogen and nitrate nitrogen were the most important driving factors for soil fungal community structure. The relative abundance of saprophytic fungi was positively and negatively correlated with soil total phosphorus and pH， respectively （P<0.05）， and the relative abundance of saprophytic fungi had a significant positive correlated with soil organic matter （P<0.01）. Stand age and soil properties play a key role in the soil fungal community construction associated with P. sylvestris plantations in the Horqin Sandy Land. From young to nearly-mature plantations， the dominant symbiotic fungi accumulation， which benefit the rapid growth of plantations. In mature and over-mature plantations， the soil fungal community structure is similar， saprophytic fungi are the dominant species， and the soil fungal community network tend to complex and stable， which contribute to the resistance improvement of plantations to changeable environments. This improved information will provide a better understanding of soil fungal functions of P. sylvestris plantations in the Horqin Sandy Land， as well as the forest and soil health from the perspective of soil fungi.

Key words: soil fungi, community structure, ecological function, stand age, Pinus sylvestris var. mongolica

CLC Number:

S791.253

Dandan Li, Jiawen Li, Guanglei Gao, Ying Zhang, Yue Ren, Ye Liu, Peishan Zhao. Soil fungal community structure and functional characteristics associated with Pinussylvestris var. mongolica plantations in the Horqin Sandy Land[J]. Journal of Desert Research, 2023, 43(4): 241-251.

Figures/Tables 9

References 35

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样地	林龄/a	平均树高/m	平均胸径/cm	郁闭度
幼龄林	13	3.36±3.36	7.66±1.36	0.96
中龄林	22	5.24±0.35	12.42±1.56	0.88
近熟林	34	9.61±0.98	15.71±2.35	0.85
成熟林	41	10.39±0.85	23.01±2.86	0.81
过熟林	55	11.35±0.91	25.96±3.64	0.79

样地	林龄/a	平均树高/m	平均胸径/cm	郁闭度
幼龄林	13	3.36±3.36	7.66±1.36	0.96
中龄林	22	5.24±0.35	12.42±1.56	0.88
近熟林	34	9.61±0.98	15.71±2.35	0.85
成熟林	41	10.39±0.85	23.01±2.86	0.81
过熟林	55	11.35±0.91	25.96±3.64	0.79

样地	多样性指数
样地	Shannon	Simpson	Pielou
幼龄林	1.89±0.26^a	0.87±0.19^a	0.47±0.09^b
中龄林	1.64±0.23^b	0.66±0.16^b	0.65±0.04^a
近熟林	1.83±0.15^b	0.82±0.08^b	0.52±0.05^a
成熟林	1.59±0.29^b	0.68±0.13^b	0.51±0.07^a
过熟林	1.61±0.22^b	0.66±0.13^b	0.49±0.06^a
F	1.48	1.97	1.85
P	0.24	0.14	0.16

样地	多样性指数
样地	Shannon	Simpson	Pielou
幼龄林	1.89±0.26^a	0.87±0.19^a	0.47±0.09^b
中龄林	1.64±0.23^b	0.66±0.16^b	0.65±0.04^a
近熟林	1.83±0.15^b	0.82±0.08^b	0.52±0.05^a
成熟林	1.59±0.29^b	0.68±0.13^b	0.51±0.07^a
过熟林	1.61±0.22^b	0.66±0.13^b	0.49±0.06^a
F	1.48	1.97	1.85
P	0.24	0.14	0.16

拓扑特征	林龄
拓扑特征	幼龄林	中龄林	近熟林	成熟林	过熟林
点	719	691	665	769	784
边	10 202	12 709	884	14 060	15 862
平均度	28.378	36.784	26.598	36.567	40.464
平均路径长度	2.237	6.113	3.009	2.692	3.339
平均聚类系数	0.960	0.943	0.972	0.973	0.951
模块化	0.761	0.712	0.775	0.755	0.706
正相关	93.37%	97.61%	97.08%	98.36%	97.98%

Soil fungal community structure and functional characteristics associated with Pinussylvestris var. mongolica plantations in the Horqin Sandy Land

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项目	幼龄林	中龄林	近熟林	成熟林	过熟林
碱解氮/（mg·kg^-1）	26.27±6.64^c	34.14±1.66^b	28.66±1.64^c	40.67±1.84^a	26.20±6.62^c
铵态氮/（mg·kg^-1）	1.53±0.20^ab	1.52±0.11^ab	1.32±0.19^b	1.72±0.18^a	1.71±0.22^a
硝态氮/（mg·kg^-1）	1.18±0.31^b	2.84±0.59^a	2.49±0.63^a	3.07±0.63^a	1.70±0.39^b
有效磷/（mg·kg^-1）	0.78±0.39^b	1.36±16^a	0.86±0.11^b	0.82±0.10^b	0.82±0.19^b
全磷/%	0.16±0.03^bc	0.21±0.01^a	0.16±0.01^bc	0.17±0.01^b	0.15±0.01^c
全钾/%	21.11±0.90^a	20.14±0.99^ab	19.42±0.83^b	20.34±0.95^ab	20.77±0.74^a
有机质/（g·kg^-1）	8.42±2.11^b	11.84±0.59^a	9.04±0.57^b	12.14±0.86^a	8.47±0.54^b
pH值	5.14±0.24^b	6.53±0.41^a	6.69±0.32^a	6.30±0.43^a	4.50±0.18^c
土壤含水量/%	7.11±0.42^b	5.44±0.25^c	6.65±0.56^b	8.07±0.40^a	7.16±0.53^b
毛管孔隙度/%	31.36±1.61^a	28.77±2.05^a	30.45±3.91^a	32.00±4.73^a	27.01±3.91^a

项目	多样性指数			营养型
项目	Shannon	Simpson	Pielou	共生营养型	腐生营养型	病理营养型
碱解氮/（mg·kg^-1）	-0.421	-0.159	-0.398	0.218	-0.826	0.769
铵态氮/（mg·kg^-1）	-0.240	-0.358	-0.096	0.473	-0.782	0.698
硝态氮/（mg·kg^-1）	0.587	0.764	0.523	0.564	-0.892	0.815
有效磷/（mg·kg^-1）	-0.462	-0.247	-0.485	0.781	-0.483	0.091
全磷/%	-0.240	-0.384	-0.548	-0.521	-0.624	0.979*
全钾/%	-0.258	-0.256	-0.379	-0.075	-0.773	0.927
有机质/（g·kg^-1）	0.074	0.068	0.125	-0.346	0.997**	-0.679
pH值	0.645	0.729	0.831	0.437	0.874	-0.976*
土壤含水量/%	0.954	0.534	0.962	0.035	-0.902	0.875
毛管孔隙度/%	0.598	0.376	0.251	0.241	-0.670	-0.651

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