The succession of sand-binding forest impacts the construction effect of sand fixation forest. With the method of substituting time with space, the artificial sand-binding forests afforested for 5, 15 and 25 years at the southern edge of Tengger Desert were selected as a success stage series, and the adjacent mobile sand dune and native vegetation habitat were took as the reference sites to reveal the ecological mechanism of the succession of artificial sand-binding forests from the aspects of ecological hydrology, soil seed bank and soil-vegetation corresponding relationship, which provided theoretical basis for the construction, protection and restoration of regional artificial sand-binding vegetation. The results showed that during the successional process of artificial sand-binding forest of Hedysarum scoparium in the southern edge of Tengger Desert, the artificial population of H. scoparium, pioneer plants Agriophyllum squarrosum and Artemisia sphaerocephala declined gradually, and the natural dominant species changed from annual herbs to semi shrubs, then to semi shrubs and perennial herbs. After 25 years, a relatively stable near natural sand-binding vegetation similar to the zonal vegetation up to 50% was formed, which showed a trend of grassland desertification succession. During the successional process of artificial sand-binding forest, the topsoil water holding capacity increased continually, reaching 3.4 times of that of the mobile sand dunes after 25 years, which resulted in the shallow distribution of soil moisture and the drought of the deep soil, driving the decline of the deep root plants and the development of the shallow root herbs. A. squarrosum and A. sphaerocephala in the soil seed bank decreased rapidly and disappeared after 25 years; A. ordosica continued to increase and Stipa breviflora appeared after 25 years, which was more than 50% similar to the aboveground vegetation composition, driving the artificial sand-binding forest to A. ordosica vegetation with perennial herbs. With the succession of artificial sand-binding forest, soil bulk density continued to decrease, clay, silt, organic matter and total nitrogen continued to increase, which was closely related to the community and species composition of artificial sand-binding forest, and determined the succession stage and species composition of sand-binding forest. Obviously, sand fixation and afforestation is a necessary measure for ecological restoration of the desertified land in arid areas, which starts and accelerates the restoration and succession of natural vegetation. However, the zonal vegetation and soil habitat conditions should be fully considered in sand fixation and afforestation, so as to establish stable near natural vegetation quickly.